Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin αs

Ryosuke Ushijima, Naoko Sakaguchi, Arihiro Kano, Atsushi Maruyama, Yoichi Miyamoto, Toshihiro Sekimoto, Yoshihiro Yoneda, Kenji Ogino, Taro Tachibana

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Abstract

The signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is involved in a variety of biological functions. STAT3 is activated by cytokines and growth factors via the phosphorylation of a tyrosine residue, dimerization, and subsequent nuclear translocation. However, the mechanism of its nuclear translocation is unclear. A study of the cytokine-stimulated import of STAT3 into the nucleus is reported herein. An oncostatin M (OSM)-dependent nuclear import assay system was first established in living cells. Using this system, we demonstrated that the microinjection of the importin α5/NPI-1 mutant, an anti-importin β antibody, and the RanQ69L mutant inhibited the nuclear import of STAT3. Second, we showed that tyrosine-phosphorylated STAT3 associates, not only with importin α5/NPI-1 but also with other importin αs, as a result of OSM stimulation, as evidenced by a solution binding assay. These findings suggest that the extracellular signal-dependent nuclear transport of STAT3 is mediated by various importin αs, importin β, and Ran.

Original languageEnglish
Pages (from-to)880-886
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume330
Issue number3
DOIs
Publication statusPublished - May 13 2005

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Karyopherins
STAT3 Transcription Factor
Cell Nucleus Active Transport
Oncostatin M
Tyrosine
Assays
Cytokines
Phosphorylation
Dimerization
Microinjections
Anti-Idiotypic Antibodies
Intercellular Signaling Peptides and Proteins
Transcription Factors
Cells
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin αs. / Ushijima, Ryosuke; Sakaguchi, Naoko; Kano, Arihiro; Maruyama, Atsushi; Miyamoto, Yoichi; Sekimoto, Toshihiro; Yoneda, Yoshihiro; Ogino, Kenji; Tachibana, Taro.

In: Biochemical and Biophysical Research Communications, Vol. 330, No. 3, 13.05.2005, p. 880-886.

Research output: Contribution to journalArticle

Ushijima, R, Sakaguchi, N, Kano, A, Maruyama, A, Miyamoto, Y, Sekimoto, T, Yoneda, Y, Ogino, K & Tachibana, T 2005, 'Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin αs', Biochemical and Biophysical Research Communications, vol. 330, no. 3, pp. 880-886. https://doi.org/10.1016/j.bbrc.2005.03.063
Ushijima, Ryosuke ; Sakaguchi, Naoko ; Kano, Arihiro ; Maruyama, Atsushi ; Miyamoto, Yoichi ; Sekimoto, Toshihiro ; Yoneda, Yoshihiro ; Ogino, Kenji ; Tachibana, Taro. / Extracellular signal-dependent nuclear import of STAT3 is mediated by various importin αs. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 330, No. 3. pp. 880-886.
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