TY - GEN
T1 - Extract of radical species by circulating reactive interface of microfluidic chip for protein crystallization
AU - Kobayashi, Takuya
AU - Ohtonari, Keishi
AU - Yamanishi, Yoko
N1 - Funding Information:
This work was partly financed by ministry of education, culture, sports, science and technology (25289059, 25108504, 15H00903 and 25630091) and Japan Science and Technology Agency (JST) PRESTO program. Authors thanked to Dr. Nakai at JEOL Ltd. for valuable advices related to ESR and Aquatech co., Ltd. for valuable advices related to pump operation.
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - The paper reports radical-assisted protein crystallization by using circulating reactive interface of microfluidic chip. The novelty of our work is separation and extraction of radical species from plasma irradiation and to produce reactive interface in micro-fluidic chip. It was also confirmed that several radicals species are detected by ESR (Electron Spin Resonance) measurement and the radical-induced protein crystal are produced for low concentration of protein. This microfluidic chip contributes to the protein crystal production and bio-medical applications.
AB - The paper reports radical-assisted protein crystallization by using circulating reactive interface of microfluidic chip. The novelty of our work is separation and extraction of radical species from plasma irradiation and to produce reactive interface in micro-fluidic chip. It was also confirmed that several radicals species are detected by ESR (Electron Spin Resonance) measurement and the radical-induced protein crystal are produced for low concentration of protein. This microfluidic chip contributes to the protein crystal production and bio-medical applications.
UR - http://www.scopus.com/inward/record.url?scp=84983326058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983326058&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983326058
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 251
EP - 253
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -