Extraction behavior of hemoglobin using reversed micelles by dioleyl phosphoric acid

Tsutomu Ono, Masahiro Goto, Fumiyuki Nakashio, T. Alan Hatton

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A new surfactant, dioleyl phosphoric acid (DOLPA), has been applied to the extraction of hemoglobin using reversed micelles. The reversed micelles formed by DOLPA can easily extract hemoglobin from aqueous to reversed micellar solutions. DOLPA is the first surfactant to extract hemoglobin completely without using any cosurfactants. On the basis of the difference between DOLPA and AOT reversed micelles in the forward extraction behavior of hemoglobin, the nature of the interfacial complex that would be formed between surfactants and hemoglobin at the oil-water interface was found to be the dominant factor in determining the extraction efficiency of hemoglobin by reversed micelles. In addition, back-transfer studies of hemoglobin from the DOLPA reversed micelles were also carried out by the phase transfer method. It was found that hemoglobin, once dissolved into the DOLPA reversed micelles, is not transferred to a fresh aqueous solution even when the conditions are adjusted to not allow the forward transfer of hemoglobin. However, the addition of several kinds of alcohol drastically improved the yield in the back-transfer of hemoglobin. The efficiency in the back-transfer of hemoglobin strongly depends on the aqueous conditions that are in contact with the reversed micelles, such as pH, ionic strength, and alcohol concentration. A pH higher than the pI of hemoglobin, a salt concentration lower than that of the water pool, and the proper concentration of alcohol are required for the recovery aqueous phase to ensure the back-transfer of hemoglobin from the DOLPA reversed micelles.

Original languageEnglish
Pages (from-to)793-800
Number of pages8
JournalBiotechnology Progress
Volume12
Issue number6
DOIs
Publication statusPublished - Nov 1 1996

Fingerprint

phosphoric acid
Micelles
micelles
hemoglobin
Hemoglobins
Surface-Active Agents
surfactants
alcohols
Alcohols
dioleoyl phosphate
water
oil-water interface
Water
extracts
ionic strength
salt concentration
Osmolar Concentration
aqueous solutions
Oils
Salts

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

Extraction behavior of hemoglobin using reversed micelles by dioleyl phosphoric acid. / Ono, Tsutomu; Goto, Masahiro; Nakashio, Fumiyuki; Hatton, T. Alan.

In: Biotechnology Progress, Vol. 12, No. 6, 01.11.1996, p. 793-800.

Research output: Contribution to journalArticle

Ono, Tsutomu ; Goto, Masahiro ; Nakashio, Fumiyuki ; Hatton, T. Alan. / Extraction behavior of hemoglobin using reversed micelles by dioleyl phosphoric acid. In: Biotechnology Progress. 1996 ; Vol. 12, No. 6. pp. 793-800.
@article{792257b749ca4b24be4cc8c6a83a1266,
title = "Extraction behavior of hemoglobin using reversed micelles by dioleyl phosphoric acid",
abstract = "A new surfactant, dioleyl phosphoric acid (DOLPA), has been applied to the extraction of hemoglobin using reversed micelles. The reversed micelles formed by DOLPA can easily extract hemoglobin from aqueous to reversed micellar solutions. DOLPA is the first surfactant to extract hemoglobin completely without using any cosurfactants. On the basis of the difference between DOLPA and AOT reversed micelles in the forward extraction behavior of hemoglobin, the nature of the interfacial complex that would be formed between surfactants and hemoglobin at the oil-water interface was found to be the dominant factor in determining the extraction efficiency of hemoglobin by reversed micelles. In addition, back-transfer studies of hemoglobin from the DOLPA reversed micelles were also carried out by the phase transfer method. It was found that hemoglobin, once dissolved into the DOLPA reversed micelles, is not transferred to a fresh aqueous solution even when the conditions are adjusted to not allow the forward transfer of hemoglobin. However, the addition of several kinds of alcohol drastically improved the yield in the back-transfer of hemoglobin. The efficiency in the back-transfer of hemoglobin strongly depends on the aqueous conditions that are in contact with the reversed micelles, such as pH, ionic strength, and alcohol concentration. A pH higher than the pI of hemoglobin, a salt concentration lower than that of the water pool, and the proper concentration of alcohol are required for the recovery aqueous phase to ensure the back-transfer of hemoglobin from the DOLPA reversed micelles.",
author = "Tsutomu Ono and Masahiro Goto and Fumiyuki Nakashio and Hatton, {T. Alan}",
year = "1996",
month = "11",
day = "1",
doi = "10.1021/bp960055y",
language = "English",
volume = "12",
pages = "793--800",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - Extraction behavior of hemoglobin using reversed micelles by dioleyl phosphoric acid

AU - Ono, Tsutomu

AU - Goto, Masahiro

AU - Nakashio, Fumiyuki

AU - Hatton, T. Alan

PY - 1996/11/1

Y1 - 1996/11/1

N2 - A new surfactant, dioleyl phosphoric acid (DOLPA), has been applied to the extraction of hemoglobin using reversed micelles. The reversed micelles formed by DOLPA can easily extract hemoglobin from aqueous to reversed micellar solutions. DOLPA is the first surfactant to extract hemoglobin completely without using any cosurfactants. On the basis of the difference between DOLPA and AOT reversed micelles in the forward extraction behavior of hemoglobin, the nature of the interfacial complex that would be formed between surfactants and hemoglobin at the oil-water interface was found to be the dominant factor in determining the extraction efficiency of hemoglobin by reversed micelles. In addition, back-transfer studies of hemoglobin from the DOLPA reversed micelles were also carried out by the phase transfer method. It was found that hemoglobin, once dissolved into the DOLPA reversed micelles, is not transferred to a fresh aqueous solution even when the conditions are adjusted to not allow the forward transfer of hemoglobin. However, the addition of several kinds of alcohol drastically improved the yield in the back-transfer of hemoglobin. The efficiency in the back-transfer of hemoglobin strongly depends on the aqueous conditions that are in contact with the reversed micelles, such as pH, ionic strength, and alcohol concentration. A pH higher than the pI of hemoglobin, a salt concentration lower than that of the water pool, and the proper concentration of alcohol are required for the recovery aqueous phase to ensure the back-transfer of hemoglobin from the DOLPA reversed micelles.

AB - A new surfactant, dioleyl phosphoric acid (DOLPA), has been applied to the extraction of hemoglobin using reversed micelles. The reversed micelles formed by DOLPA can easily extract hemoglobin from aqueous to reversed micellar solutions. DOLPA is the first surfactant to extract hemoglobin completely without using any cosurfactants. On the basis of the difference between DOLPA and AOT reversed micelles in the forward extraction behavior of hemoglobin, the nature of the interfacial complex that would be formed between surfactants and hemoglobin at the oil-water interface was found to be the dominant factor in determining the extraction efficiency of hemoglobin by reversed micelles. In addition, back-transfer studies of hemoglobin from the DOLPA reversed micelles were also carried out by the phase transfer method. It was found that hemoglobin, once dissolved into the DOLPA reversed micelles, is not transferred to a fresh aqueous solution even when the conditions are adjusted to not allow the forward transfer of hemoglobin. However, the addition of several kinds of alcohol drastically improved the yield in the back-transfer of hemoglobin. The efficiency in the back-transfer of hemoglobin strongly depends on the aqueous conditions that are in contact with the reversed micelles, such as pH, ionic strength, and alcohol concentration. A pH higher than the pI of hemoglobin, a salt concentration lower than that of the water pool, and the proper concentration of alcohol are required for the recovery aqueous phase to ensure the back-transfer of hemoglobin from the DOLPA reversed micelles.

UR - http://www.scopus.com/inward/record.url?scp=0030294676&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030294676&partnerID=8YFLogxK

U2 - 10.1021/bp960055y

DO - 10.1021/bp960055y

M3 - Article

C2 - 8983206

AN - SCOPUS:0030294676

VL - 12

SP - 793

EP - 800

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 6

ER -