Extraction of DNA by reversed micelles

Masahiro Goto, Tsutomu Ono, Akihiko Horiuchi, Shintaro Furusaki

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    Deoxyribonucleotide (DNA) from salmon testes was successfully extracted by reversed micelles that are formed by a cationic surfactant in isooctane. The degree of extraction of DNA was strongly influenced by the type of surfactant in the organic solvent and ionic strength in the material aqueous phase. The driving force of the DNA transfer was electrostatic interaction between cationic surfactants and negatively charged DNA. Cationic surfactants possessing two long-alkyl chains are one of the best candidates for the successful transfer of DNA from an aqueous solution to a reversed micellar solution.

    Original languageEnglish
    Pages (from-to)123-125
    Number of pages3
    JournalJournal of Chemical Engineering of Japan
    Volume32
    Issue number1
    DOIs
    Publication statusPublished - 1999

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)
    • Chemical Engineering(all)

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