Extremely high expression of β-actin mRNA in osteoclasts resorbing alveolar bone located at the distal area of the developing molar tooth germ in newborn rats

Toshio Kukita, Akiko Kukita, Licin Xu, Kazuko Toh, Quanyong Tang, Hisayuki Nomiyama, Tadahiko Iijima

Research output: Contribution to journalArticle

Abstract

Expression of β-actin is widely utilized as an internal control of mRNA expression in various cells. Here we show evidence that the expression level of β-actin mRNA in osteoclasts significantly differs in its intensity according to the position in bone tissues. By use of in situ hybridization, we obtained clear data showing that osteoclasts facing the distal part of a developing molar tooth germ expressed extremely high levels of β-actin mRNA in comparison with other osteoclasts observed in the mandibular bone surface. No signal was detected when β-nerve growth factor transcripts were observed. Electron microscopic analysis revealed that osteoclasts localized in these areas were functionally active, estimated from the expression of high levels of the osteoclast membrane antigen, Katl antigen, which associated with active osteoclasts. These data suggest that osteoclasts expressing extremely high levels of β-actin are highly related to active osteoclasts induced by the mechanical stress caused by physiological movement of molar tooth germ towards distal directions in the developing mandible.

Original languageEnglish
Pages (from-to)545-550
Number of pages6
JournalJournal of Electron Microscopy
Volume52
Issue number6
DOIs
Publication statusPublished - Dec 1 2003

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Tooth Germ
Osteoclasts
teeth
bones
rats
Rats
Actins
Bone
Bone and Bones
Messenger RNA
antigens
nerves
Antigens
membranes
Mechanical Stress
Nerve Growth Factor
cells
Mandible
In Situ Hybridization
electrons

All Science Journal Classification (ASJC) codes

  • Instrumentation

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Extremely high expression of β-actin mRNA in osteoclasts resorbing alveolar bone located at the distal area of the developing molar tooth germ in newborn rats. / Kukita, Toshio; Kukita, Akiko; Xu, Licin; Toh, Kazuko; Tang, Quanyong; Nomiyama, Hisayuki; Iijima, Tadahiko.

In: Journal of Electron Microscopy, Vol. 52, No. 6, 01.12.2003, p. 545-550.

Research output: Contribution to journalArticle

Kukita, Toshio ; Kukita, Akiko ; Xu, Licin ; Toh, Kazuko ; Tang, Quanyong ; Nomiyama, Hisayuki ; Iijima, Tadahiko. / Extremely high expression of β-actin mRNA in osteoclasts resorbing alveolar bone located at the distal area of the developing molar tooth germ in newborn rats. In: Journal of Electron Microscopy. 2003 ; Vol. 52, No. 6. pp. 545-550.
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