Fabrication and evaluation of carbonate apatite-coated calcium carbonate bone substitutes for bone tissue engineering

Masako Fujioka-Kobayashi, Kanji Tsuru, Hirokazu Nagai, Kenji Fujisawa, Takaharu Kudoh, Go Ohe, Kunio Ishikawa, Youji Miyamoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Carbonate apatite-coated calcium carbonate (CO3Ap/CaCO3) was fabricated through a dissolution–precipitation reaction using CaCO3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO3Ap shell, along with Ca2+ release from the CaCO3 core and quicker resorption of the CaCO3 core. In the present study, CaCO3, 10% CO3Ap/CaCO3, 30% CO3Ap/CaCO3, and CO3Ap granules were fabricated and examined histologically to evaluate their potential as bone substitutes. Larger contents of CaCO3 in the granules resulted in higher Ca2+ release and promoted cell proliferation of murine preosteoblasts at 6 days compared with CO3Ap. Interestingly, in a rabbit femur defect model, 10% CO3Ap/CaCO3 induced significantly higher new bone formation and higher material resorption compared with CO3Ap at 8 weeks. Nevertheless, CO3Ap showed a superior osteoconductive potential compared with 10% CO3Ap/CaCO3 at 8 weeks. All tested granules were most likely resorbed by cell mediation including multinucleated giant cell functions. Therefore, we conclude that CO3Ap/CaCO3 has a positive potential for bone tissue engineering based on well-controlled calcium release, bone formation, and material resorption.

Original languageEnglish
Pages (from-to)2077-2087
Number of pages11
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number10
DOIs
Publication statusPublished - Oct 1 2018

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Bone Substitutes
Calcium Carbonate
Apatite
Calcium carbonate
Tissue Engineering
Tissue engineering
Osteogenesis
Carbonates
Bone
Bone and Bones
Fabrication
Giant Cells
Femur
Cell Proliferation
Rabbits
Calcium
Bioelectric potentials
Cell proliferation
Defects
carboapatite

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Fabrication and evaluation of carbonate apatite-coated calcium carbonate bone substitutes for bone tissue engineering. / Fujioka-Kobayashi, Masako; Tsuru, Kanji; Nagai, Hirokazu; Fujisawa, Kenji; Kudoh, Takaharu; Ohe, Go; Ishikawa, Kunio; Miyamoto, Youji.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 10, 01.10.2018, p. 2077-2087.

Research output: Contribution to journalArticle

Fujioka-Kobayashi, Masako ; Tsuru, Kanji ; Nagai, Hirokazu ; Fujisawa, Kenji ; Kudoh, Takaharu ; Ohe, Go ; Ishikawa, Kunio ; Miyamoto, Youji. / Fabrication and evaluation of carbonate apatite-coated calcium carbonate bone substitutes for bone tissue engineering. In: Journal of Tissue Engineering and Regenerative Medicine. 2018 ; Vol. 12, No. 10. pp. 2077-2087.
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