TY - CHAP
T1 - Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars
AU - Kasama, Toshihiro
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
N1 - Funding Information:
This work was partially supported by the Japan Society for the Promotion of Science (JSPS, grant no. 24245013). We would like to thank Kansai Paint Co., Ltd., for valuable discussions.
Publisher Copyright:
© Springer Science+Business Media LLC 2017.
PY - 2017
Y1 - 2017
N2 - Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.
AB - Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.
UR - http://www.scopus.com/inward/record.url?scp=85008452835&partnerID=8YFLogxK
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U2 - 10.1007/978-1-4939-6734-6_4
DO - 10.1007/978-1-4939-6734-6_4
M3 - Chapter
C2 - 28044286
AN - SCOPUS:85008452835
T3 - Methods in Molecular Biology
SP - 49
EP - 56
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -