Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars

Toshihiro Kasama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages49-56
Number of pages8
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1547
ISSN (Print)1064-3745

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Lab-On-A-Chip Devices
Immobilized Antibodies
Hydrogel
Microspheres
Immunoassay
Immunochromatography
Equipment and Supplies
Agglutination
Latex
C-Reactive Protein
Enzyme-Linked Immunosorbent Assay
Antigens
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Kasama, T., Kaji, N., Tokeshi, M., & Baba, Y. (2017). Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars. In Methods in Molecular Biology (pp. 49-56). (Methods in Molecular Biology; Vol. 1547). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6734-6_4

Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars. / Kasama, Toshihiro; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 49-56 (Methods in Molecular Biology; Vol. 1547).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kasama, T, Kaji, N, Tokeshi, M & Baba, Y 2017, Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1547, Humana Press Inc., pp. 49-56. https://doi.org/10.1007/978-1-4939-6734-6_4
Kasama T, Kaji N, Tokeshi M, Baba Y. Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 49-56. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6734-6_4
Kasama, Toshihiro ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 49-56 (Methods in Molecular Biology).
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