Fabrication of 3D tissue-like structure using magnetite nanoparticles and magnetic force

Akira Ito, Kousuke Ino, Kazunori Shimizu, Hiroyuki Honda, Masamichi Kamihira

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Novel technologies to establish 3D tissue-like constructs are desired for tissue engineering. In the present study, magnetic force and magnetite nanoparticles were used to construct a layered mesenchymal stem cell (MSC) sheet, a layered cardiomyocyte sheet, and a layered fibroblast sheet involving capillaries. Magnetite cationic liposomes (MCLs) were taken up by the target cells. When a magnet was set under a tissue culture dish, magnetically labeled target cells were attracted and then adhered to form a layered cell sheet. MSC sheets were harvested and transplanted into the bone defect in cranium of nude rats, resulted in formation of new bones surrounded by osteoblast-like cells. For cardiomyocyte sheets, the immunofluorescence staining revealed the existence of gap junctions within the cardiomyocyte sheets. For fibroblast sheets, normal human dermal fibroblasts (NHDFs) sheets contained the major dermal extracellular matrix components. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NIIDF sheets, resulted in tube-like formation of HUVECs. These results suggest that this novel use of magnetite nanoparticles and magnetic force, which we refer to as "magnetic force-based tissue engineering (Mag-TE)", offers a major advancement in tissue engineering.

Original languageEnglish
Title of host publication2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
DOIs
Publication statusPublished - Dec 1 2006
Event2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS - Nagoya, Japan
Duration: Nov 5 2006Nov 8 2006

Publication series

Name2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

Other

Other2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
CountryJapan
CityNagoya
Period11/5/0611/8/06

All Science Journal Classification (ASJC) codes

  • Computational Mechanics
  • Control and Systems Engineering
  • Human Factors and Ergonomics

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  • Cite this

    Ito, A., Ino, K., Shimizu, K., Honda, H., & Kamihira, M. (2006). Fabrication of 3D tissue-like structure using magnetite nanoparticles and magnetic force. In 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS [4110394] (2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS). https://doi.org/10.1109/MHS.2006.320291