Fabrication of a portable electrochemiluminescence-induced fluorescence detection chip with microfluidic excitation source for point-of-care diagnostics

Miho Tsuwaki, Takashi Kasahara, Tomohiko Edura, Juro Oshima, Eri Kunisawa, Ryoichi Ishimatsu, Shigeyuki Matsunami, Toshihiko Imato, Chihaya Adachi, Shuichi Shoji, Jun Mizuno

Research output: Contribution to journalArticle

Abstract

In this study, we propose an electrochemiluminescence (ECL)-induced fluorescence detection chip toward portable point-of-care diagnostics. A prototype chip consists of polydimethylsiloxane (PDMS)-based flow channels for target samples and on-demand multi-color microfluidic excitation sources. The microfluidic ECL cells were fabricated via a heterogeneous bonding technique through the use of self-assembled monolayers. The flow channels for target samples, which were formed by soft lithography, were combined with the excitation source. A pink fluorescence of resorufin was successfully detected with yellow ECL emission of 5,6,11,12-tetraphenyltetracene (rubrene). The proposed principle can open a new possibility for on-onsite multiple fluorescence detections.

Original languageEnglish
Pages (from-to)230-235
Number of pages6
JournalIEEJ Transactions on Sensors and Micromachines
Volume135
Issue number6
DOIs
Publication statusPublished - Jun 1 2015

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Microfluidics
Fluorescence
Channel flow
Fabrication
Self assembled monolayers
Polydimethylsiloxane
Lithography
Color

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Fabrication of a portable electrochemiluminescence-induced fluorescence detection chip with microfluidic excitation source for point-of-care diagnostics. / Tsuwaki, Miho; Kasahara, Takashi; Edura, Tomohiko; Oshima, Juro; Kunisawa, Eri; Ishimatsu, Ryoichi; Matsunami, Shigeyuki; Imato, Toshihiko; Adachi, Chihaya; Shoji, Shuichi; Mizuno, Jun.

In: IEEJ Transactions on Sensors and Micromachines, Vol. 135, No. 6, 01.06.2015, p. 230-235.

Research output: Contribution to journalArticle

Tsuwaki, Miho ; Kasahara, Takashi ; Edura, Tomohiko ; Oshima, Juro ; Kunisawa, Eri ; Ishimatsu, Ryoichi ; Matsunami, Shigeyuki ; Imato, Toshihiko ; Adachi, Chihaya ; Shoji, Shuichi ; Mizuno, Jun. / Fabrication of a portable electrochemiluminescence-induced fluorescence detection chip with microfluidic excitation source for point-of-care diagnostics. In: IEEJ Transactions on Sensors and Micromachines. 2015 ; Vol. 135, No. 6. pp. 230-235.
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