TY - JOUR
T1 - Fabrication of dicalcium phosphate dihydrate-coated β-TCP granules and evaluation of their osteoconductivity using experimental rats
AU - Shariff, Khairul Anuar
AU - Tsuru, Kanji
AU - Ishikawa, Kunio
N1 - Funding Information:
This study was supported, in part, by the Strategic Promotion of Innovative Research and Development Program (15im0502004h0004) from Japan Agency for Medical Research and Development. The first author would like to thank AUN-SEED/Net, JICA, Universiti Sains Malaysia (USM), and the Ministry of Education, Malaysia, for their financial support.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - β-Tricalcium phosphate (β-TCP) has attracted much attention as an artificial bone substitute owing to its biocompatibility and osteoconductivity. In this study, osteoconductivity of β-TCP bone substitute was enhanced without using growth factors or cells. Dicalcium phosphate dihydrate (DCPD), which is known to possess the highest solubility among calcium phosphates, was coated on β-TCP granules by exposing their surface with acidic calcium phosphate solution. The amount of coated DCPD was regulated by changing the reaction time between β-TCP granules and acidic calcium phosphate solution. Histomorphometry analysis obtained from histological results revealed that the approximately 10 mol% DCPD-coated β-TCP granules showed the largest new bone formation compared to DCPD-free β-TCP granules, approximately 2.5 mol% DCPD-coated β-TCP granules, or approximately 27 mol% DCPD-coated β-TCP granules after 2 and 4 weeks of implantation. Based on this finding, we demonstrate that the osteoconductivity of β-TCP granules could be improved by coating their surface with an appropriate amount of DCPD.
AB - β-Tricalcium phosphate (β-TCP) has attracted much attention as an artificial bone substitute owing to its biocompatibility and osteoconductivity. In this study, osteoconductivity of β-TCP bone substitute was enhanced without using growth factors or cells. Dicalcium phosphate dihydrate (DCPD), which is known to possess the highest solubility among calcium phosphates, was coated on β-TCP granules by exposing their surface with acidic calcium phosphate solution. The amount of coated DCPD was regulated by changing the reaction time between β-TCP granules and acidic calcium phosphate solution. Histomorphometry analysis obtained from histological results revealed that the approximately 10 mol% DCPD-coated β-TCP granules showed the largest new bone formation compared to DCPD-free β-TCP granules, approximately 2.5 mol% DCPD-coated β-TCP granules, or approximately 27 mol% DCPD-coated β-TCP granules after 2 and 4 weeks of implantation. Based on this finding, we demonstrate that the osteoconductivity of β-TCP granules could be improved by coating their surface with an appropriate amount of DCPD.
UR - http://www.scopus.com/inward/record.url?scp=85015668509&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015668509&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2017.03.004
DO - 10.1016/j.msec.2017.03.004
M3 - Article
C2 - 28415432
AN - SCOPUS:85015668509
VL - 75
SP - 1411
EP - 1419
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -