Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages425-441
Number of pages17
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1416
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Kuboki, T., & Kidoaki, S. (2016). Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture. In Methods in Molecular Biology (pp. 425-441). (Methods in Molecular Biology; Vol. 1416). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3584-0_25