Fabrication of liver-derived extracellular matrix nanofibers and functional evaluation in in vitro culture using primary hepatocytes

Ronald Bual, Haruna Kimura, Yasuhiro Ikegami, Nana Shirakigawa, Hiroyuki Ijima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fabrication of functional scaffolds is one of the main goals of tissue engineering, particularly scaffolds mimicking the native extracellular matrix (ECM) and stimulating a microenvironment that supports cell growth and function. In this study, a scaffold was developed using liver-derived ECM (L-ECM), gelatin, and polycaprolactone (PCL) by conventional electrospinning. This study aimed to improve the gelatin-PCL nanofiber blend by incorporating L-ECM. The morphology of the nanofibrous scaffold was investigated by scanning electron microscopy. Characterizations such as Fourier transform infrared spectroscopy (FTIR), tensile strength test, and water contact angle analysis revealed the favorable characteristics of the scaffold. Particularly, the FTIR spectra confirmed the presence of L-ECM, gelatin, and PCL, even after crosslinking treatment, while tensile strength analysis revealed suitable mechanical properties and water contact angle measurement showed the improved hydrophilic characteristics of the scaffold. The biocompatibility of the composite nanofibers was evaluated by in vitro culture of primary hepatocytes. Phase-contrast microscope images verified that the L-ECM nanofibers improved hepatocyte adhesion and facilitated the formation of a tissue-like structure. Moreover, high liver-specific functions were obtained in L-ECM nanofibers compared to gelatin/PCL nanofibers. Thus, L-ECM nanofibers can be used in tissue engineering, particularly in liver regeneration.

Original languageEnglish
Pages (from-to)518-528
Number of pages11
JournalMaterialia
Volume4
DOIs
Publication statusPublished - Dec 1 2018

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Nanofibers
Liver
Military electronic countermeasures
Scaffolds (biology)
Fabrication
Polycaprolactone
Gelatin
Tissue engineering
Contact angle
Fourier transform infrared spectroscopy
Tensile strength
Hepatocytes
Water
Electrospinning
Cell growth
Angle measurement
Biocompatibility
Scaffolds
Crosslinking
Microscopes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Fabrication of liver-derived extracellular matrix nanofibers and functional evaluation in in vitro culture using primary hepatocytes. / Bual, Ronald; Kimura, Haruna; Ikegami, Yasuhiro; Shirakigawa, Nana; Ijima, Hiroyuki.

In: Materialia, Vol. 4, 01.12.2018, p. 518-528.

Research output: Contribution to journalArticle

Bual, Ronald ; Kimura, Haruna ; Ikegami, Yasuhiro ; Shirakigawa, Nana ; Ijima, Hiroyuki. / Fabrication of liver-derived extracellular matrix nanofibers and functional evaluation in in vitro culture using primary hepatocytes. In: Materialia. 2018 ; Vol. 4. pp. 518-528.
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