Fabrication of novel molecular recognition membranes by physical adsorption and self-assembly for surface plasmon resonance detection of TNT

Dhesingh Ravi Shankaran, Toshikazu Kawaguchi, Sook Jin Kim, Kiyoshi Matsumoto, Kiyoshi Toko, Norio Miura

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Recent concern on international terrorism and weapons of mass destruction demands the development of novel analytical methods for identification and quantification of explosive molecules. In this article, we describe the development of high-performance immunosensors for detection of 2,4,6-trinitrotoluene (TNT), a prime component of the landmines and bombs used by terrorist and military forces. The immunosensors were constructed by physical adsorption and self-assembly methods, and their binding interactions with a monoclonal anti-TNT antibody were evaluated for TNT detection using the surface plasmon resonance technique. A home-made 2,4,6-trinitrophenyl-keyhole limpet hemocyanine conjugate was used for physical adsorption. A poly(ethylene glycol) hydrazine hydrochloride thiolate was used in the construction of self-assembled monolayer surface and was immobilized with trinitrophenyl-β-alanine by the amide coupling method. The immunosensors were highly selective, regenerable, rapid, and exhibited remarkable sensitivity down to the parts-per-trillion level for TNT by the indirect competitive inhibition principle.

Original languageEnglish
Pages (from-to)771-781
Number of pages11
JournalInternational Journal of Environmental Analytical Chemistry
Volume87
Issue number10-11
DOIs
Publication statusPublished - Aug 1 2007

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Science(all)
  • Environmental Chemistry

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