Fabrication of scaffold-free contractile skeletal muscle tissue using magnetite-incorporated myogenic C2C12 cells

Hideaki Fujita, Kazunori Shimizu, Yasunori Yamamoto, Akira Ito, Masamichi Kamihira, Eiji Nagamori

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have fabricated a functional skeletal muscle tissue using magnetite-incorporated myogenic cell line C2C12 and a magnetic field. Magnetite-incorporated C2C12 cells were patterned linearly on a monolayer of fibroblast NIH3T3 cells, using a magnetic field concentrator. After induction of differentiation, the C2C12 cells fused and formed multi-nucleated myotubes. The 3T3 layer became detached in a sheet-like manner after cultivation in differentiation medium for 5-8 days. When two separate collagen films were placed on a culture dish as tendon structures, a cylindrical construct was formed. Histological observation of the fabricated cylindrical tissue revealed the presence of multinucleate cells within it. Immunofluorescence staining of the construct showed the presence of sarcomere structures within the construct. Western blot analysis showed that muscle proteins were expressed in the construct. When the construct was stimulated with electric pulses, it exhibited active tension of approximately 1 μN. These results demonstrate that functional skeletal muscle tissue was formed through magnetic force-based tissue engineering. This is the first report of fabrication of skeletal muscle tissue with active tension-generating capability using magnetic force-based tissue engineering. The scaffold-free skeletal muscle tissue engineering technique presented in this study will be useful for regenerative medicine, drug screening or use as a bio-actuator.

Original languageEnglish
Pages (from-to)437-443
Number of pages7
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume4
Issue number6
DOIs
Publication statusPublished - Aug 2010

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Ferrosoferric Oxide
Magnetite
Scaffolds
Muscle
Skeletal Muscle
Tissue Engineering
Tissue engineering
Tissue
Fabrication
Muscles
Magnetic Fields
Magnetic fields
Sarcomeres
Preclinical Drug Evaluations
Regenerative Medicine
Muscle Proteins
Tendons
Skeletal Muscle Fibers
Fibroblasts
Scaffolds (biology)

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Fabrication of scaffold-free contractile skeletal muscle tissue using magnetite-incorporated myogenic C2C12 cells. / Fujita, Hideaki; Shimizu, Kazunori; Yamamoto, Yasunori; Ito, Akira; Kamihira, Masamichi; Nagamori, Eiji.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 4, No. 6, 08.2010, p. 437-443.

Research output: Contribution to journalArticle

Fujita, Hideaki ; Shimizu, Kazunori ; Yamamoto, Yasunori ; Ito, Akira ; Kamihira, Masamichi ; Nagamori, Eiji. / Fabrication of scaffold-free contractile skeletal muscle tissue using magnetite-incorporated myogenic C2C12 cells. In: Journal of Tissue Engineering and Regenerative Medicine. 2010 ; Vol. 4, No. 6. pp. 437-443.
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