Facile distinction of neutral and acidic tetraether lipids in archaea membrane by halogen atom adduct ions in electrospray ionization mass spectrometry

Tatsushi Murae, Yuichiro Takamatsu, Ryohei Muraoka, Satoshi Endoh, Noriaki Yamauchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Calditocaldarchaeol (neutral tetraether lipid) from Sulfolobus acidocaldarius (acidothermophilic archaea) and intact total lipid from the thermoacidophilic archaea Sulfolobus sp. was examined by electrospray ionization time-of-flight mass spectrometry in the negative-ion mode using high resolution. When the sample was injected as a solution in a 3:1 mixture of methanol (MeOH) and chloroform (CHCl3) using an infusion system, the total ether lipid afforded molecular-related ions as [M - H]- for acidic polar lipids containing a phosphoric or sulfuric group, and as [M + Cl]- ion for neutral glycolipids. The attachment of chloride was confirmed by the observation of [M + Br]- ion, instead of [M + Cl]- ion, when a 3:1 mixture of MeOH and CHBr3 was used in place of MeOH-CHCl3 as the solvent. The composition of tetraether neutral glycolipids that are different from each other only in the number of five-membered rings in the isoprenoid chain was determined on the basis of the isotope-resolved mass spectrum of [M + Cl]- ions. As for acidic tetraether lipids, molecular-related ions [M - H]-) were not observed when the 3:1 MeOH-CHBr3 mixture was used as the solvent. These results together afforded a facile method of distinguishing neutral from acidic tetraether lipids in intact total lipids of acidothermophilic archaea. This method was applied to determine the difference of the number of five-membered rings in isoprenyl chains of neutral tetraether glycolipids yielded by the Sulfolobus sp. grown at different temperatures. Discrimination of neutral tetraether glycolipids from acidic tetraether lipids in the total lipids obtained from Thermoplasma sp. was also achieved by this method.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalJournal of Mass Spectrometry
Volume37
Issue number2
DOIs
Publication statusPublished - Mar 7 2002

Fingerprint

Electrospray ionization
Halogens
Electrospray Ionization Mass Spectrometry
Archaea
Mass spectrometry
Ions
Membranes
Lipids
Atoms
Glycolipids
Sulfolobus
Sulfolobus acidocaldarius
Thermoplasma
Terpenes
Chloroform
Isotopes
Ether
Methanol
Chlorides
Mass Spectrometry

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Facile distinction of neutral and acidic tetraether lipids in archaea membrane by halogen atom adduct ions in electrospray ionization mass spectrometry. / Murae, Tatsushi; Takamatsu, Yuichiro; Muraoka, Ryohei; Endoh, Satoshi; Yamauchi, Noriaki.

In: Journal of Mass Spectrometry, Vol. 37, No. 2, 07.03.2002, p. 209-215.

Research output: Contribution to journalArticle

@article{9ddecae018354b66961fb2016f63a463,
title = "Facile distinction of neutral and acidic tetraether lipids in archaea membrane by halogen atom adduct ions in electrospray ionization mass spectrometry",
abstract = "Calditocaldarchaeol (neutral tetraether lipid) from Sulfolobus acidocaldarius (acidothermophilic archaea) and intact total lipid from the thermoacidophilic archaea Sulfolobus sp. was examined by electrospray ionization time-of-flight mass spectrometry in the negative-ion mode using high resolution. When the sample was injected as a solution in a 3:1 mixture of methanol (MeOH) and chloroform (CHCl3) using an infusion system, the total ether lipid afforded molecular-related ions as [M - H]- for acidic polar lipids containing a phosphoric or sulfuric group, and as [M + Cl]- ion for neutral glycolipids. The attachment of chloride was confirmed by the observation of [M + Br]- ion, instead of [M + Cl]- ion, when a 3:1 mixture of MeOH and CHBr3 was used in place of MeOH-CHCl3 as the solvent. The composition of tetraether neutral glycolipids that are different from each other only in the number of five-membered rings in the isoprenoid chain was determined on the basis of the isotope-resolved mass spectrum of [M + Cl]- ions. As for acidic tetraether lipids, molecular-related ions [M - H]-) were not observed when the 3:1 MeOH-CHBr3 mixture was used as the solvent. These results together afforded a facile method of distinguishing neutral from acidic tetraether lipids in intact total lipids of acidothermophilic archaea. This method was applied to determine the difference of the number of five-membered rings in isoprenyl chains of neutral tetraether glycolipids yielded by the Sulfolobus sp. grown at different temperatures. Discrimination of neutral tetraether glycolipids from acidic tetraether lipids in the total lipids obtained from Thermoplasma sp. was also achieved by this method.",
author = "Tatsushi Murae and Yuichiro Takamatsu and Ryohei Muraoka and Satoshi Endoh and Noriaki Yamauchi",
year = "2002",
month = "3",
day = "7",
doi = "10.1002/jms.276",
language = "English",
volume = "37",
pages = "209--215",
journal = "Journal of Mass Spectrometry",
issn = "1076-5174",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Facile distinction of neutral and acidic tetraether lipids in archaea membrane by halogen atom adduct ions in electrospray ionization mass spectrometry

AU - Murae, Tatsushi

AU - Takamatsu, Yuichiro

AU - Muraoka, Ryohei

AU - Endoh, Satoshi

AU - Yamauchi, Noriaki

PY - 2002/3/7

Y1 - 2002/3/7

N2 - Calditocaldarchaeol (neutral tetraether lipid) from Sulfolobus acidocaldarius (acidothermophilic archaea) and intact total lipid from the thermoacidophilic archaea Sulfolobus sp. was examined by electrospray ionization time-of-flight mass spectrometry in the negative-ion mode using high resolution. When the sample was injected as a solution in a 3:1 mixture of methanol (MeOH) and chloroform (CHCl3) using an infusion system, the total ether lipid afforded molecular-related ions as [M - H]- for acidic polar lipids containing a phosphoric or sulfuric group, and as [M + Cl]- ion for neutral glycolipids. The attachment of chloride was confirmed by the observation of [M + Br]- ion, instead of [M + Cl]- ion, when a 3:1 mixture of MeOH and CHBr3 was used in place of MeOH-CHCl3 as the solvent. The composition of tetraether neutral glycolipids that are different from each other only in the number of five-membered rings in the isoprenoid chain was determined on the basis of the isotope-resolved mass spectrum of [M + Cl]- ions. As for acidic tetraether lipids, molecular-related ions [M - H]-) were not observed when the 3:1 MeOH-CHBr3 mixture was used as the solvent. These results together afforded a facile method of distinguishing neutral from acidic tetraether lipids in intact total lipids of acidothermophilic archaea. This method was applied to determine the difference of the number of five-membered rings in isoprenyl chains of neutral tetraether glycolipids yielded by the Sulfolobus sp. grown at different temperatures. Discrimination of neutral tetraether glycolipids from acidic tetraether lipids in the total lipids obtained from Thermoplasma sp. was also achieved by this method.

AB - Calditocaldarchaeol (neutral tetraether lipid) from Sulfolobus acidocaldarius (acidothermophilic archaea) and intact total lipid from the thermoacidophilic archaea Sulfolobus sp. was examined by electrospray ionization time-of-flight mass spectrometry in the negative-ion mode using high resolution. When the sample was injected as a solution in a 3:1 mixture of methanol (MeOH) and chloroform (CHCl3) using an infusion system, the total ether lipid afforded molecular-related ions as [M - H]- for acidic polar lipids containing a phosphoric or sulfuric group, and as [M + Cl]- ion for neutral glycolipids. The attachment of chloride was confirmed by the observation of [M + Br]- ion, instead of [M + Cl]- ion, when a 3:1 mixture of MeOH and CHBr3 was used in place of MeOH-CHCl3 as the solvent. The composition of tetraether neutral glycolipids that are different from each other only in the number of five-membered rings in the isoprenoid chain was determined on the basis of the isotope-resolved mass spectrum of [M + Cl]- ions. As for acidic tetraether lipids, molecular-related ions [M - H]-) were not observed when the 3:1 MeOH-CHBr3 mixture was used as the solvent. These results together afforded a facile method of distinguishing neutral from acidic tetraether lipids in intact total lipids of acidothermophilic archaea. This method was applied to determine the difference of the number of five-membered rings in isoprenyl chains of neutral tetraether glycolipids yielded by the Sulfolobus sp. grown at different temperatures. Discrimination of neutral tetraether glycolipids from acidic tetraether lipids in the total lipids obtained from Thermoplasma sp. was also achieved by this method.

UR - http://www.scopus.com/inward/record.url?scp=0036180172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036180172&partnerID=8YFLogxK

U2 - 10.1002/jms.276

DO - 10.1002/jms.276

M3 - Article

C2 - 11857765

AN - SCOPUS:0036180172

VL - 37

SP - 209

EP - 215

JO - Journal of Mass Spectrometry

JF - Journal of Mass Spectrometry

SN - 1076-5174

IS - 2

ER -