Facile method for the preparation of lyso-GM1 and lyso-GM2

Takayuki Ando, Su Chen Li, Makoto Ito, Yu Teh Li

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

This paper reports a facile method for the preparation of lyso-GM1 [Galβ1 → 3GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-sphingosine] and lyso-GM2 [GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-sphingosine], respectively, from GM1 [Galβ1 → 3GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-Cer] and GM2 [GalNAcβ1 → 4(Neu5Acα2 → 3)Galβ1 → 4Glcβ1 → 1′-Cer], using sphingolipid ceramide deacylase and high performance anion-exchange chromatography (HPAEC). The enzymatically released lyso-GM1 and/or lyso-GM2 was effectively separated from its parent ganglioside by HPAEC using a Mono Q HR 5/5 column with an Amersham Biosciences fast protein liquid chromatography system. The yield was almost quantitative and the separation completed in approximately 3 h. This method is more convenient and effective than the conventional method using alkaline hydrolysis and silicic acid chromatography to generate and purify lyso-gangliosides.

Original languageEnglish
Pages (from-to)193-195
Number of pages3
JournalJournal of Chromatography A
Volume1078
Issue number1-2
DOIs
Publication statusPublished - Jun 17 2005

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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