Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor

Cuicui Li, Masafumi Takeo, Masayoshi Matsuda, Hiroko Nagai, Sun Xizheng, Wataru Hatanaka, Akihiro Kishimura, Hiroyuki Inoue, Kenzaburo Tani, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticle

Abstract

The introduction of proteins into dendritic cells (DCs) ex vivo is a critical step for the DC-based immunotherapy of cancer. Here, we developed a biotin-modified polymer with multiple hydrophobic membrane anchors for cells that functions as a synthetic receptor for an antigen protein, ovalbumin (OVA), to introduce it efficiently into DCs compared with the conventional pulsing method. Our method showed significant advantages, including the rapid incorporation of OVA and the activation of antigen-specific T cells in a MHC-restricted manner. When mice were immunized by DCs treated with our method, tumor growth was completely suppressed, indicating that our method can be used to prepare adjuvant DCs.

Original languageEnglish
Pages (from-to)1207-1212
Number of pages6
JournalMedChemComm
Volume8
Issue number6
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Artificial Receptors
Antigen Presentation
Immunotherapy
Dendritic Cells
Polymers
Antigens
Peptides
Ovalbumin
Neoplasms
CD27 Antigens
Synthetic Vaccines
T-cells
Biotin
Anchors
Tumors
Proteins
Chemical activation
Cell Membrane
Membranes
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Cite this

Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor. / Li, Cuicui; Takeo, Masafumi; Matsuda, Masayoshi; Nagai, Hiroko; Xizheng, Sun; Hatanaka, Wataru; Kishimura, Akihiro; Inoue, Hiroyuki; Tani, Kenzaburo; Mori, Takeshi; Katayama, Yoshiki.

In: MedChemComm, Vol. 8, No. 6, 01.01.2017, p. 1207-1212.

Research output: Contribution to journalArticle

Li, Cuicui ; Takeo, Masafumi ; Matsuda, Masayoshi ; Nagai, Hiroko ; Xizheng, Sun ; Hatanaka, Wataru ; Kishimura, Akihiro ; Inoue, Hiroyuki ; Tani, Kenzaburo ; Mori, Takeshi ; Katayama, Yoshiki. / Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor. In: MedChemComm. 2017 ; Vol. 8, No. 6. pp. 1207-1212.
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