Facilitation of proteasomal degradation of p27Kip1 by N-terminal cleavage and their sequence requirements

Katsuya Hirano, Eikichi Ihara, Mayumi Hirano, Junji Nishimura, Hajime Nawata, Hideo Kanaide

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The sequence requirement for N-terminal cleavage and the proteasomal degradation of p27Kip1 and their relationship was investigated. Residues 5-8 were required for the cleavage and the mutation of S10 to E inhibited the cleavage. The C-terminal PEST sequence was necessary for the degradation and residue R165 was found to play an important role in the degradation. The inhibition of the cleavage by deleting residues 5-8 inhibited the degradation, while the fragment mimicking the cleavage product accelerated the degradation. Both the cleavage and degradation demonstrated a similar sensitivity toward proteasome inhibitors and ATP depletion. These two processes are thus suggested to be tightly linked and sequential.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalFEBS Letters
Issue number1-3
Publication statusPublished - Sep 10 2004

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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