Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1

Miyako Nagamachi, Daiji Sakata, Kenji Kabashima, Tomoyuki Furuyashiki, Takahiko Murata, Eri Segi-Nishida, Kitipong Soontrapa, Toshiyuki Matsuoka, Yoshiki Miyachi, Shuh Narumiya

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Abstract

Prostaglandin E2 (PGE2) exerts its actions via four subtypes of the PGE receptor, EP1-4. We show that mice deficient in EP1 exhibited significantly attenuated Th1 response in contact hypersensitivity induced by dinitrofluorobenzene (DNFB). This phenotype was recapitulated in wild-type mice by administration of an EP1-selective antagonist during the sensitization phase, and by adoptive transfer of T cells from sensitized EP1-/- mice. Conversely, an EP1-selective agonist facilitated Th1 differentiation of naive T cells in vitro. Finally, CD11c+ cells containing the inducible form of PGE synthase increased in number in the draining lymph nodes after DNFB application. These results suggest that PGE 2 produced by dendritic cells in the lymph nodes acts on EP1 in naive T cells to promote Th1 differentiation. JEM

Original languageEnglish
Pages (from-to)2865-2874
Number of pages10
JournalJournal of Experimental Medicine
Volume204
Issue number12
DOIs
Publication statusPublished - Nov 26 2007

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Nagamachi, M., Sakata, D., Kabashima, K., Furuyashiki, T., Murata, T., Segi-Nishida, E., ... Narumiya, S. (2007). Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1. Journal of Experimental Medicine, 204(12), 2865-2874. https://doi.org/10.1084/jem.20070773