Factor B polymorphism in four Asian populations

Electrophoretic characterization of a subtype of “F” and of new variants in the Chinese

Koichi Suzuki, Preeyachit Charoenwongse, Augusto L. Lingao, Nafisa M. Contractor, Sho Matsushita, Takehiko Sasazuki, Hideo Matsumoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The genetic polymorphism of factor B (BF) in four Asian populations was studied by immunofixation after agarose gel electrophoresis (AGE) in both veronal buffer (VB) and Tris/glycine/veronal buffer (TGVB). A suballele of the BF*F and three rare alleles were detected in a Chinese population. The subtype and the rare variants were further characterized by using isoelectric focusing in polyacrylamide gels (PAGIF) and by zymosan treatment of the serum samples. The subtype was identified as BFFb1, first defined in the Japanese and found to occur at a polymorphic frequency. One of the rare variants exhibited an intermediate mobility between BFF and BFS and seemed to be similar to BFF025. PAGIF, however, could clearly discriminate the two variants that had a similar mobility on AGE. The results obtained in this study suggest that conventional electrophoresis is not enough to characterize a new variant and to discriminate it from previously reported variants. The findings obtained on immunofixation after AGE/TGVB and in PAGIF must be added to those obtained by the standard method.

Original languageEnglish
Pages (from-to)486-489
Number of pages4
JournalELECTROPHORESIS
Volume8
Issue number10
DOIs
Publication statusPublished - Jan 1 1987

Fingerprint

Barbital
Agar Gel Electrophoresis
Electrophoresis
Polymorphism
Sepharose
Glycine
Gels
Buffers
Population
Tromethamine
Zymosan
Isoelectric Focusing
Genetic Polymorphisms
Alleles
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry

Cite this

Suzuki, K., Charoenwongse, P., Lingao, A. L., Contractor, N. M., Matsushita, S., Sasazuki, T., & Matsumoto, H. (1987). Factor B polymorphism in four Asian populations: Electrophoretic characterization of a subtype of “F” and of new variants in the Chinese. ELECTROPHORESIS, 8(10), 486-489. https://doi.org/10.1002/elps.1150081006

Factor B polymorphism in four Asian populations : Electrophoretic characterization of a subtype of “F” and of new variants in the Chinese. / Suzuki, Koichi; Charoenwongse, Preeyachit; Lingao, Augusto L.; Contractor, Nafisa M.; Matsushita, Sho; Sasazuki, Takehiko; Matsumoto, Hideo.

In: ELECTROPHORESIS, Vol. 8, No. 10, 01.01.1987, p. 486-489.

Research output: Contribution to journalArticle

Suzuki, K, Charoenwongse, P, Lingao, AL, Contractor, NM, Matsushita, S, Sasazuki, T & Matsumoto, H 1987, 'Factor B polymorphism in four Asian populations: Electrophoretic characterization of a subtype of “F” and of new variants in the Chinese', ELECTROPHORESIS, vol. 8, no. 10, pp. 486-489. https://doi.org/10.1002/elps.1150081006
Suzuki, Koichi ; Charoenwongse, Preeyachit ; Lingao, Augusto L. ; Contractor, Nafisa M. ; Matsushita, Sho ; Sasazuki, Takehiko ; Matsumoto, Hideo. / Factor B polymorphism in four Asian populations : Electrophoretic characterization of a subtype of “F” and of new variants in the Chinese. In: ELECTROPHORESIS. 1987 ; Vol. 8, No. 10. pp. 486-489.
@article{485c8a64ddab46b5886504a7a5a079c3,
title = "Factor B polymorphism in four Asian populations: Electrophoretic characterization of a subtype of “F” and of new variants in the Chinese",
abstract = "The genetic polymorphism of factor B (BF) in four Asian populations was studied by immunofixation after agarose gel electrophoresis (AGE) in both veronal buffer (VB) and Tris/glycine/veronal buffer (TGVB). A suballele of the BF*F and three rare alleles were detected in a Chinese population. The subtype and the rare variants were further characterized by using isoelectric focusing in polyacrylamide gels (PAGIF) and by zymosan treatment of the serum samples. The subtype was identified as BFFb1, first defined in the Japanese and found to occur at a polymorphic frequency. One of the rare variants exhibited an intermediate mobility between BFF and BFS and seemed to be similar to BFF025. PAGIF, however, could clearly discriminate the two variants that had a similar mobility on AGE. The results obtained in this study suggest that conventional electrophoresis is not enough to characterize a new variant and to discriminate it from previously reported variants. The findings obtained on immunofixation after AGE/TGVB and in PAGIF must be added to those obtained by the standard method.",
author = "Koichi Suzuki and Preeyachit Charoenwongse and Lingao, {Augusto L.} and Contractor, {Nafisa M.} and Sho Matsushita and Takehiko Sasazuki and Hideo Matsumoto",
year = "1987",
month = "1",
day = "1",
doi = "10.1002/elps.1150081006",
language = "English",
volume = "8",
pages = "486--489",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "10",

}

TY - JOUR

T1 - Factor B polymorphism in four Asian populations

T2 - Electrophoretic characterization of a subtype of “F” and of new variants in the Chinese

AU - Suzuki, Koichi

AU - Charoenwongse, Preeyachit

AU - Lingao, Augusto L.

AU - Contractor, Nafisa M.

AU - Matsushita, Sho

AU - Sasazuki, Takehiko

AU - Matsumoto, Hideo

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The genetic polymorphism of factor B (BF) in four Asian populations was studied by immunofixation after agarose gel electrophoresis (AGE) in both veronal buffer (VB) and Tris/glycine/veronal buffer (TGVB). A suballele of the BF*F and three rare alleles were detected in a Chinese population. The subtype and the rare variants were further characterized by using isoelectric focusing in polyacrylamide gels (PAGIF) and by zymosan treatment of the serum samples. The subtype was identified as BFFb1, first defined in the Japanese and found to occur at a polymorphic frequency. One of the rare variants exhibited an intermediate mobility between BFF and BFS and seemed to be similar to BFF025. PAGIF, however, could clearly discriminate the two variants that had a similar mobility on AGE. The results obtained in this study suggest that conventional electrophoresis is not enough to characterize a new variant and to discriminate it from previously reported variants. The findings obtained on immunofixation after AGE/TGVB and in PAGIF must be added to those obtained by the standard method.

AB - The genetic polymorphism of factor B (BF) in four Asian populations was studied by immunofixation after agarose gel electrophoresis (AGE) in both veronal buffer (VB) and Tris/glycine/veronal buffer (TGVB). A suballele of the BF*F and three rare alleles were detected in a Chinese population. The subtype and the rare variants were further characterized by using isoelectric focusing in polyacrylamide gels (PAGIF) and by zymosan treatment of the serum samples. The subtype was identified as BFFb1, first defined in the Japanese and found to occur at a polymorphic frequency. One of the rare variants exhibited an intermediate mobility between BFF and BFS and seemed to be similar to BFF025. PAGIF, however, could clearly discriminate the two variants that had a similar mobility on AGE. The results obtained in this study suggest that conventional electrophoresis is not enough to characterize a new variant and to discriminate it from previously reported variants. The findings obtained on immunofixation after AGE/TGVB and in PAGIF must be added to those obtained by the standard method.

UR - http://www.scopus.com/inward/record.url?scp=84988073684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84988073684&partnerID=8YFLogxK

U2 - 10.1002/elps.1150081006

DO - 10.1002/elps.1150081006

M3 - Article

VL - 8

SP - 486

EP - 489

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 10

ER -