Failure of t cell receptor negative selection in murine intestinal intra-epitheliallymphocytes

Shinji Murosaki, Yasunobu Yoshikai, Atsunori Ishida, Takao Nakamura, Goro Matsuzaki, Hiroaki Taki Moto, Hiroyuki Yuuki, Kikuo Nomoto

    Research output: Contribution to journalArticle

    59 Citations (Scopus)

    Abstract

    The intestinal intra-epithelial lymphocytes (IEL) are divided into several subsets on the basis of expression of T cell receptor (TCR) a/3 and y6, intensity of Thy-1 expression and expression ofLyt-3 chain. To investigate the differentiation pathway of the IEL, we examined the repertoire of\f0 segments of T cells in the IEL in BALB/c (H-2d, Mls-1b2a) or AKR/J (H-2k, Mls-1a2b) mice. Among freshly isolated IEL, an appreciable number of T cells bearing Vβ3 or Vβ11, which recognize Mls-2a- or MHC IE-encoded molecules respectively, were detected in BALB/c mice.Similarly, in AKR/J mice, IEL contained appreciable levels of V^6-bearing T cells. Vβ3- orVβ11 -bearing T cells in the IEL in BALB/c mice increased to a significant level when incubatedwith staphylococcal enterotoxin A which specifically stimulates V03- and Vg11 -bearing T cells.Most of IEL without clonal deletion expressed Lyt-2 but not Lyt-3 antigens. Such T ceils werehardly detected in other organs, including liver. Our results indicate that TCRa/3-bearing intestinalIEL that have not undergone negative selection may have differentiated outside the thymus, presumably at a local site of the intestine and can respond normally to the signal via their TCR.

    Original languageEnglish
    Pages (from-to)1005-1013
    Number of pages9
    JournalInternational Immunology
    Volume3
    Issue number10
    DOIs
    Publication statusPublished - Oct 1991

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    Lymphocytes
    T-Lymphocytes
    T-Cell Antigen Receptor
    Clonal Deletion
    Inbred AKR Mouse
    Lymphocyte Count
    Thymus Gland
    Intestines
    Antigens
    Liver

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Murosaki, S., Yoshikai, Y., Ishida, A., Nakamura, T., Matsuzaki, G., Taki Moto, H., ... Nomoto, K. (1991). Failure of t cell receptor negative selection in murine intestinal intra-epitheliallymphocytes. International Immunology, 3(10), 1005-1013. https://doi.org/10.1093/intimm/3.10.1005

    Failure of t cell receptor negative selection in murine intestinal intra-epitheliallymphocytes. / Murosaki, Shinji; Yoshikai, Yasunobu; Ishida, Atsunori; Nakamura, Takao; Matsuzaki, Goro; Taki Moto, Hiroaki; Yuuki, Hiroyuki; Nomoto, Kikuo.

    In: International Immunology, Vol. 3, No. 10, 10.1991, p. 1005-1013.

    Research output: Contribution to journalArticle

    Murosaki, S, Yoshikai, Y, Ishida, A, Nakamura, T, Matsuzaki, G, Taki Moto, H, Yuuki, H & Nomoto, K 1991, 'Failure of t cell receptor negative selection in murine intestinal intra-epitheliallymphocytes', International Immunology, vol. 3, no. 10, pp. 1005-1013. https://doi.org/10.1093/intimm/3.10.1005
    Murosaki S, Yoshikai Y, Ishida A, Nakamura T, Matsuzaki G, Taki Moto H et al. Failure of t cell receptor negative selection in murine intestinal intra-epitheliallymphocytes. International Immunology. 1991 Oct;3(10):1005-1013. https://doi.org/10.1093/intimm/3.10.1005
    Murosaki, Shinji ; Yoshikai, Yasunobu ; Ishida, Atsunori ; Nakamura, Takao ; Matsuzaki, Goro ; Taki Moto, Hiroaki ; Yuuki, Hiroyuki ; Nomoto, Kikuo. / Failure of t cell receptor negative selection in murine intestinal intra-epitheliallymphocytes. In: International Immunology. 1991 ; Vol. 3, No. 10. pp. 1005-1013.
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