FANCD2 mRNA overexpression is a bona fide indicator of lymph node metastasis in human colorectal cancer

Heita Ozawa, Masaaki Iwatsuki, Koshi Mimori, Tetsuya Sato, Fredrik Johansson, Hiroyuki Toh, Masahiko Watanabe, Masaki Mori

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Lymph node metastasis is widely accepted as one of the most important determinants of prognosis in colorectal cancer (CRC) patients. Therefore, there is an urgent need to identify molecular markers that can be used to predict lymph node metastasis. Materials and Methods: Candidate genes were found using LMD and cDNA microarrays in a large-scale study of CRC, followed by Penalized Canonical Correlation Analysis (PCCA). We focused on the Fanconi anemia, complementation group D2 (FANCD2) gene and evaluated FANCD2 mRNA expression in 133 CRC cases to determine the clinicopathological significance of FANCD2 expression. Results: The mean level of FANCD2 mRNA expression in tumor tissue specimens was significantly higher than in nontumor tissue. FANCD2 expression was found to be a significant factor affecting lymph node metastasis: the high FANCD2 expression group had a significantly poorer prognosis than the low expression group. Conclusions: This study suggests that PCCA can be used to identify genes related to clinicopathological features. Furthermore, high FANCD2 expression was a significant independent factor for lymph node metastasis.

Original languageEnglish
Pages (from-to)2341-2348
Number of pages8
JournalAnnals of Surgical Oncology
Volume17
Issue number9
DOIs
Publication statusPublished - Sep 1 2010

Fingerprint

Fanconi Anemia
Colorectal Neoplasms
Lymph Nodes
Neoplasm Metastasis
Messenger RNA
Genes
Oligonucleotide Array Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

FANCD2 mRNA overexpression is a bona fide indicator of lymph node metastasis in human colorectal cancer. / Ozawa, Heita; Iwatsuki, Masaaki; Mimori, Koshi; Sato, Tetsuya; Johansson, Fredrik; Toh, Hiroyuki; Watanabe, Masahiko; Mori, Masaki.

In: Annals of Surgical Oncology, Vol. 17, No. 9, 01.09.2010, p. 2341-2348.

Research output: Contribution to journalArticle

Ozawa, Heita ; Iwatsuki, Masaaki ; Mimori, Koshi ; Sato, Tetsuya ; Johansson, Fredrik ; Toh, Hiroyuki ; Watanabe, Masahiko ; Mori, Masaki. / FANCD2 mRNA overexpression is a bona fide indicator of lymph node metastasis in human colorectal cancer. In: Annals of Surgical Oncology. 2010 ; Vol. 17, No. 9. pp. 2341-2348.
@article{aa0eba80c03542f0bd8bfec2e42e5334,
title = "FANCD2 mRNA overexpression is a bona fide indicator of lymph node metastasis in human colorectal cancer",
abstract = "Background: Lymph node metastasis is widely accepted as one of the most important determinants of prognosis in colorectal cancer (CRC) patients. Therefore, there is an urgent need to identify molecular markers that can be used to predict lymph node metastasis. Materials and Methods: Candidate genes were found using LMD and cDNA microarrays in a large-scale study of CRC, followed by Penalized Canonical Correlation Analysis (PCCA). We focused on the Fanconi anemia, complementation group D2 (FANCD2) gene and evaluated FANCD2 mRNA expression in 133 CRC cases to determine the clinicopathological significance of FANCD2 expression. Results: The mean level of FANCD2 mRNA expression in tumor tissue specimens was significantly higher than in nontumor tissue. FANCD2 expression was found to be a significant factor affecting lymph node metastasis: the high FANCD2 expression group had a significantly poorer prognosis than the low expression group. Conclusions: This study suggests that PCCA can be used to identify genes related to clinicopathological features. Furthermore, high FANCD2 expression was a significant independent factor for lymph node metastasis.",
author = "Heita Ozawa and Masaaki Iwatsuki and Koshi Mimori and Tetsuya Sato and Fredrik Johansson and Hiroyuki Toh and Masahiko Watanabe and Masaki Mori",
year = "2010",
month = "9",
day = "1",
doi = "10.1245/s10434-010-1002-7",
language = "English",
volume = "17",
pages = "2341--2348",
journal = "Annals of Surgical Oncology",
issn = "1068-9265",
publisher = "Springer New York",
number = "9",

}

TY - JOUR

T1 - FANCD2 mRNA overexpression is a bona fide indicator of lymph node metastasis in human colorectal cancer

AU - Ozawa, Heita

AU - Iwatsuki, Masaaki

AU - Mimori, Koshi

AU - Sato, Tetsuya

AU - Johansson, Fredrik

AU - Toh, Hiroyuki

AU - Watanabe, Masahiko

AU - Mori, Masaki

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Background: Lymph node metastasis is widely accepted as one of the most important determinants of prognosis in colorectal cancer (CRC) patients. Therefore, there is an urgent need to identify molecular markers that can be used to predict lymph node metastasis. Materials and Methods: Candidate genes were found using LMD and cDNA microarrays in a large-scale study of CRC, followed by Penalized Canonical Correlation Analysis (PCCA). We focused on the Fanconi anemia, complementation group D2 (FANCD2) gene and evaluated FANCD2 mRNA expression in 133 CRC cases to determine the clinicopathological significance of FANCD2 expression. Results: The mean level of FANCD2 mRNA expression in tumor tissue specimens was significantly higher than in nontumor tissue. FANCD2 expression was found to be a significant factor affecting lymph node metastasis: the high FANCD2 expression group had a significantly poorer prognosis than the low expression group. Conclusions: This study suggests that PCCA can be used to identify genes related to clinicopathological features. Furthermore, high FANCD2 expression was a significant independent factor for lymph node metastasis.

AB - Background: Lymph node metastasis is widely accepted as one of the most important determinants of prognosis in colorectal cancer (CRC) patients. Therefore, there is an urgent need to identify molecular markers that can be used to predict lymph node metastasis. Materials and Methods: Candidate genes were found using LMD and cDNA microarrays in a large-scale study of CRC, followed by Penalized Canonical Correlation Analysis (PCCA). We focused on the Fanconi anemia, complementation group D2 (FANCD2) gene and evaluated FANCD2 mRNA expression in 133 CRC cases to determine the clinicopathological significance of FANCD2 expression. Results: The mean level of FANCD2 mRNA expression in tumor tissue specimens was significantly higher than in nontumor tissue. FANCD2 expression was found to be a significant factor affecting lymph node metastasis: the high FANCD2 expression group had a significantly poorer prognosis than the low expression group. Conclusions: This study suggests that PCCA can be used to identify genes related to clinicopathological features. Furthermore, high FANCD2 expression was a significant independent factor for lymph node metastasis.

UR - http://www.scopus.com/inward/record.url?scp=77956343107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956343107&partnerID=8YFLogxK

U2 - 10.1245/s10434-010-1002-7

DO - 10.1245/s10434-010-1002-7

M3 - Article

C2 - 20339950

AN - SCOPUS:77956343107

VL - 17

SP - 2341

EP - 2348

JO - Annals of Surgical Oncology

JF - Annals of Surgical Oncology

SN - 1068-9265

IS - 9

ER -