FancJ/Brip1 helicase protects against genomic losses and gains in vertebrate cells

Hiroyuki Kitao, Indrajit Nanda, Ryuichi P. Sugino, Aiko Kinomura, Mitsuyoshi Yamazoe, Hiroshi Arakawa, Michael Schmid, Hideki Innan, Kevin Hiom, Minoru Takata

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Defects in the FANCJ/BRIP1 helicase gene are associated with genome instability disorders such as familial breast cancer or Fanconi anemia (FA). Although FANCJ has an in vitro activity to resolve G-quadruplex (G4) structures, and FANCJ ortholog in C. elegans prevents G4-associated deletions during replication, how FANCJ loss affects genome integrity in higher organisms remains unclear. Here, we report that FANCJ, but not other FA genes FANCD2 or FANCC, protected against large-scale genomic deletion that occurred frequently at the rearranged immunoglobulin heavy chain (IgH) locus in chicken DT40 cell line, suggesting that FancJ protects the genome independently of the FA ubiquitination pathway. In a more unbiased approach using array-comparative genomic hybridization, we identified de novo deletions as well as amplifications in fancj cells kept in culture for 2months. A cluster of G4 sequence motifs was found near the breakpoint of one amplified region, but G4 sequence motifs were not detected at the breakpoints of two deleted regions. These results collectively suggest that, unlike in C. elegans, actions of vertebrate FANCJ to promote genome stability may not be limited to protection against the G4-mediated gene deletions.

Original languageEnglish
Pages (from-to)714-727
Number of pages14
JournalGenes to Cells
Volume16
Issue number6
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Fanconi Anemia
Vertebrates
Genomic Instability
Genome
G-Quadruplexes
Immunoglobulin Heavy Chains
Comparative Genomic Hybridization
Ubiquitination
Gene Deletion
Genes
Chickens
Cell Line

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Kitao, H., Nanda, I., Sugino, R. P., Kinomura, A., Yamazoe, M., Arakawa, H., ... Takata, M. (2011). FancJ/Brip1 helicase protects against genomic losses and gains in vertebrate cells. Genes to Cells, 16(6), 714-727. https://doi.org/10.1111/j.1365-2443.2011.01523.x

FancJ/Brip1 helicase protects against genomic losses and gains in vertebrate cells. / Kitao, Hiroyuki; Nanda, Indrajit; Sugino, Ryuichi P.; Kinomura, Aiko; Yamazoe, Mitsuyoshi; Arakawa, Hiroshi; Schmid, Michael; Innan, Hideki; Hiom, Kevin; Takata, Minoru.

In: Genes to Cells, Vol. 16, No. 6, 01.06.2011, p. 714-727.

Research output: Contribution to journalArticle

Kitao, H, Nanda, I, Sugino, RP, Kinomura, A, Yamazoe, M, Arakawa, H, Schmid, M, Innan, H, Hiom, K & Takata, M 2011, 'FancJ/Brip1 helicase protects against genomic losses and gains in vertebrate cells', Genes to Cells, vol. 16, no. 6, pp. 714-727. https://doi.org/10.1111/j.1365-2443.2011.01523.x
Kitao, Hiroyuki ; Nanda, Indrajit ; Sugino, Ryuichi P. ; Kinomura, Aiko ; Yamazoe, Mitsuyoshi ; Arakawa, Hiroshi ; Schmid, Michael ; Innan, Hideki ; Hiom, Kevin ; Takata, Minoru. / FancJ/Brip1 helicase protects against genomic losses and gains in vertebrate cells. In: Genes to Cells. 2011 ; Vol. 16, No. 6. pp. 714-727.
@article{fc592417833c4cb39fde5457c994b172,
title = "FancJ/Brip1 helicase protects against genomic losses and gains in vertebrate cells",
abstract = "Defects in the FANCJ/BRIP1 helicase gene are associated with genome instability disorders such as familial breast cancer or Fanconi anemia (FA). Although FANCJ has an in vitro activity to resolve G-quadruplex (G4) structures, and FANCJ ortholog in C. elegans prevents G4-associated deletions during replication, how FANCJ loss affects genome integrity in higher organisms remains unclear. Here, we report that FANCJ, but not other FA genes FANCD2 or FANCC, protected against large-scale genomic deletion that occurred frequently at the rearranged immunoglobulin heavy chain (IgH) locus in chicken DT40 cell line, suggesting that FancJ protects the genome independently of the FA ubiquitination pathway. In a more unbiased approach using array-comparative genomic hybridization, we identified de novo deletions as well as amplifications in fancj cells kept in culture for 2months. A cluster of G4 sequence motifs was found near the breakpoint of one amplified region, but G4 sequence motifs were not detected at the breakpoints of two deleted regions. These results collectively suggest that, unlike in C. elegans, actions of vertebrate FANCJ to promote genome stability may not be limited to protection against the G4-mediated gene deletions.",
author = "Hiroyuki Kitao and Indrajit Nanda and Sugino, {Ryuichi P.} and Aiko Kinomura and Mitsuyoshi Yamazoe and Hiroshi Arakawa and Michael Schmid and Hideki Innan and Kevin Hiom and Minoru Takata",
year = "2011",
month = "6",
day = "1",
doi = "10.1111/j.1365-2443.2011.01523.x",
language = "English",
volume = "16",
pages = "714--727",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - FancJ/Brip1 helicase protects against genomic losses and gains in vertebrate cells

AU - Kitao, Hiroyuki

AU - Nanda, Indrajit

AU - Sugino, Ryuichi P.

AU - Kinomura, Aiko

AU - Yamazoe, Mitsuyoshi

AU - Arakawa, Hiroshi

AU - Schmid, Michael

AU - Innan, Hideki

AU - Hiom, Kevin

AU - Takata, Minoru

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Defects in the FANCJ/BRIP1 helicase gene are associated with genome instability disorders such as familial breast cancer or Fanconi anemia (FA). Although FANCJ has an in vitro activity to resolve G-quadruplex (G4) structures, and FANCJ ortholog in C. elegans prevents G4-associated deletions during replication, how FANCJ loss affects genome integrity in higher organisms remains unclear. Here, we report that FANCJ, but not other FA genes FANCD2 or FANCC, protected against large-scale genomic deletion that occurred frequently at the rearranged immunoglobulin heavy chain (IgH) locus in chicken DT40 cell line, suggesting that FancJ protects the genome independently of the FA ubiquitination pathway. In a more unbiased approach using array-comparative genomic hybridization, we identified de novo deletions as well as amplifications in fancj cells kept in culture for 2months. A cluster of G4 sequence motifs was found near the breakpoint of one amplified region, but G4 sequence motifs were not detected at the breakpoints of two deleted regions. These results collectively suggest that, unlike in C. elegans, actions of vertebrate FANCJ to promote genome stability may not be limited to protection against the G4-mediated gene deletions.

AB - Defects in the FANCJ/BRIP1 helicase gene are associated with genome instability disorders such as familial breast cancer or Fanconi anemia (FA). Although FANCJ has an in vitro activity to resolve G-quadruplex (G4) structures, and FANCJ ortholog in C. elegans prevents G4-associated deletions during replication, how FANCJ loss affects genome integrity in higher organisms remains unclear. Here, we report that FANCJ, but not other FA genes FANCD2 or FANCC, protected against large-scale genomic deletion that occurred frequently at the rearranged immunoglobulin heavy chain (IgH) locus in chicken DT40 cell line, suggesting that FancJ protects the genome independently of the FA ubiquitination pathway. In a more unbiased approach using array-comparative genomic hybridization, we identified de novo deletions as well as amplifications in fancj cells kept in culture for 2months. A cluster of G4 sequence motifs was found near the breakpoint of one amplified region, but G4 sequence motifs were not detected at the breakpoints of two deleted regions. These results collectively suggest that, unlike in C. elegans, actions of vertebrate FANCJ to promote genome stability may not be limited to protection against the G4-mediated gene deletions.

UR - http://www.scopus.com/inward/record.url?scp=79956308523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956308523&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2443.2011.01523.x

DO - 10.1111/j.1365-2443.2011.01523.x

M3 - Article

VL - 16

SP - 714

EP - 727

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 6

ER -