Fanconi anemia group J mutation abolishes its DNA repair function by uncoupling DNA translocation from helicase activity or disruption of protein-DNA complexes

Yuliang Wu, Joshua A. Sommers, Avvaru N. Suhasini, Thomas Leonard, Julianna S. Deakyne, Alexander V. Mazin, Kazuo Shin-ya, Hiroyuki Kitao, Robert M. Brosh

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Fanconi anemia (FA) is a genetic disease characterized by congenital abnormalities, bone marrow failure, and susceptibility to leukemia and other cancers. FANCJ, one of 13 genes linked to FA, encodes a DNA helicase proposed to operate in homologous recombination repair and replicational stress response. The pathogenic FANCJ-A349P amino acid substitution resides immediately adjacent to a highly conserved cysteine of the iron-sulfur domain. Given the genetic linkage of the FANCJ-A349P allele to FA, we investigated the effect of this particular mutation on the biochemical and cellular functions of the FANCJ protein. Purified recombinant FANCJ-A349P protein had reduced iron and was defective in coupling adenosine triphosphate (ATP) hydrolysis and translocase activity to unwinding forked duplex or G-quadruplex DNA substrates or disrupting protein-DNA complexes. The FANCJ-A349P allele failed to rescue cisplatin or telomestatin sensitivity of a FA-J null cell line as detected by cell survival or γ-H2AX foci formation. Furthermore, expression of FANCJ-A349P in a wild-type background exerted a dominant-negative effect, indicating that the mutant protein interferes with normal DNA metabolism. The ability of FANCJ to use the energy from ATP hydrolysis to produce the force required to unwind DNA or destabilize protein bound to DNA is required for its role in DNA repair.

Original languageEnglish
Pages (from-to)3780-3791
Number of pages12
JournalBlood
Volume116
Issue number19
DOIs
Publication statusPublished - Nov 11 2010
Externally publishedYes

Fingerprint

DNA Helicases
Fanconi Anemia
DNA Repair
Repair
Mutation
DNA
Proteins
Hydrolysis
Iron
Adenosine Triphosphate
Alleles
G-Quadruplexes
Recombinational DNA Repair
Null Lymphocytes
Inborn Genetic Diseases
Genetic Linkage
Mutant Proteins
Amino Acid Substitution
Cells
Sulfur

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Fanconi anemia group J mutation abolishes its DNA repair function by uncoupling DNA translocation from helicase activity or disruption of protein-DNA complexes. / Wu, Yuliang; Sommers, Joshua A.; Suhasini, Avvaru N.; Leonard, Thomas; Deakyne, Julianna S.; Mazin, Alexander V.; Shin-ya, Kazuo; Kitao, Hiroyuki; Brosh, Robert M.

In: Blood, Vol. 116, No. 19, 11.11.2010, p. 3780-3791.

Research output: Contribution to journalArticle

Wu, Yuliang ; Sommers, Joshua A. ; Suhasini, Avvaru N. ; Leonard, Thomas ; Deakyne, Julianna S. ; Mazin, Alexander V. ; Shin-ya, Kazuo ; Kitao, Hiroyuki ; Brosh, Robert M. / Fanconi anemia group J mutation abolishes its DNA repair function by uncoupling DNA translocation from helicase activity or disruption of protein-DNA complexes. In: Blood. 2010 ; Vol. 116, No. 19. pp. 3780-3791.
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