Abstract
In order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast DNA extraction and purification by Generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. With this method, 3 μL blood was enough to obtain adequate target fragments in human genes. Under the optimal conditions in each step, the sample preparation for eight fragments in β-globin gene and four fragments in ras gene could be finished within 20 min. Since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. In addition, other advantages such as fast speed and low consumption of samples were demonstrated. All these merits proved that such a combination method was of great potential for the clinical diagnostics.
| Original language | English |
|---|---|
| Pages (from-to) | 175-180 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 1106 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Feb 17 2006 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Cite this
Fast extraction, amplification and analysis of genes from human blood. / Zhang, Lihua; Dang, Fuquan; Kaji, Noritada; Baba, Yoshinobu.
In: Journal of Chromatography A, Vol. 1106, No. 1-2, 17.02.2006, p. 175-180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fast extraction, amplification and analysis of genes from human blood
AU - Zhang, Lihua
AU - Dang, Fuquan
AU - Kaji, Noritada
AU - Baba, Yoshinobu
PY - 2006/2/17
Y1 - 2006/2/17
N2 - In order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast DNA extraction and purification by Generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. With this method, 3 μL blood was enough to obtain adequate target fragments in human genes. Under the optimal conditions in each step, the sample preparation for eight fragments in β-globin gene and four fragments in ras gene could be finished within 20 min. Since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. In addition, other advantages such as fast speed and low consumption of samples were demonstrated. All these merits proved that such a combination method was of great potential for the clinical diagnostics.
AB - In order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast DNA extraction and purification by Generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. With this method, 3 μL blood was enough to obtain adequate target fragments in human genes. Under the optimal conditions in each step, the sample preparation for eight fragments in β-globin gene and four fragments in ras gene could be finished within 20 min. Since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. In addition, other advantages such as fast speed and low consumption of samples were demonstrated. All these merits proved that such a combination method was of great potential for the clinical diagnostics.
UR - http://www.scopus.com/inward/record.url?scp=31344464064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=31344464064&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2005.10.071
DO - 10.1016/j.chroma.2005.10.071
M3 - Article
C2 - 16337635
AN - SCOPUS:31344464064
VL - 1106
SP - 175
EP - 180
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -