TY - JOUR
T1 - Fast extraction, amplification and analysis of genes from human blood
AU - Zhang, Lihua
AU - Dang, Fuquan
AU - Kaji, Noritada
AU - Baba, Yoshinobu
N1 - Funding Information:
The present work is partially supported under the CREST program of the Japan Science and Technology Corporation (JST), a Grant from the New Energy and Industrial Technology Development Organization (NEDO) of the Ministry of Economy, Trade and Industry, Japan, a Grant-in-Aid for Scientific Research from the Ministry of Health and Welfare, Japan, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Technology, Japan.
PY - 2006/2/17
Y1 - 2006/2/17
N2 - In order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast DNA extraction and purification by Generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. With this method, 3 μL blood was enough to obtain adequate target fragments in human genes. Under the optimal conditions in each step, the sample preparation for eight fragments in β-globin gene and four fragments in ras gene could be finished within 20 min. Since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. In addition, other advantages such as fast speed and low consumption of samples were demonstrated. All these merits proved that such a combination method was of great potential for the clinical diagnostics.
AB - In order to shorten the time spent on the sample preparation for gene analysis, a novel method was proposed through the combination of fast DNA extraction and purification by Generation capture disk, amplification by capillary polymerase chain reaction, and confirmation of amplification products by microchip electrophoresis. With this method, 3 μL blood was enough to obtain adequate target fragments in human genes. Under the optimal conditions in each step, the sample preparation for eight fragments in β-globin gene and four fragments in ras gene could be finished within 20 min. Since all the experiments were performed on commercial instruments, this method showed a wide range of applicability. In addition, other advantages such as fast speed and low consumption of samples were demonstrated. All these merits proved that such a combination method was of great potential for the clinical diagnostics.
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U2 - 10.1016/j.chroma.2005.10.071
DO - 10.1016/j.chroma.2005.10.071
M3 - Article
C2 - 16337635
AN - SCOPUS:31344464064
VL - 1106
SP - 175
EP - 180
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -