Fast-to-slow shift of muscle fiber-type composition by dietary apple polyphenols in rats: Impact of the low-dose supplementation

Wataru Mizunoya, Shinpei Okamoto, Hideo Miyahara, Mariko Akahoshi, Takahiro Suzuki, Mai Khoi Q. Do, Hideaki Ohtsubo, Yusuke Komiya, Mulan Qahar, Toshiaki Waga, Koichi Nakazato, Yoshihide Ikeuchi, Judy E. Anderson, Ryuichi Tatsumi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Our previous studies demonstrated that an 8-week intake of 5% (w/w) apple polyphenol (APP) in the diet improves muscle endurance of young-adult rats. In order to identify a lower limit of the dietary contribution of APP to the effect, the experiments were designed for lower-dose supplementation (8-week feeding of 0.5% APP in AIN-93G diet) to 12-week-old male Sprague-Dawley rats. Results clearly showed that the 0.5% APP diet significantly up-regulates slower myosin-heavy-chain (MyHC) isoform ratios (IIx and IIa relative to total MyHC) and myoglobin expression in lower hind-limb muscles examined (P < 0.05). There was a trend to increased fatigue resistance detected from measurements of relative isometric plantar-flexion force torque generated by a stimulus train delivered to the tibial nerve (F(98, 1372) = 1.246, P = 0.0574). Importantly, there was no significant difference in the animal body-phenotypes or locomotor activity shown as total moving distance in light and dark periods. Therefore, the present study encourages the notion that even low APP-intake may increase the proportions of fatigue-resistant myofibers, and has promise as a strategy for modifying performance in human sports and improving function in age-related muscle atrophy.

Original languageEnglish
Pages (from-to)489-499
Number of pages11
JournalAnimal Science Journal
Volume88
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Dietary Fiber
Malus
Polyphenols
muscle fibers
polyphenols
apples
Muscles
rats
dosage
Myosin Heavy Chains
myosin heavy chains
Diet
Fatigue
diet
Tibial Nerve
muscles
muscular atrophy
Muscular Atrophy
myoglobin
Myoglobin

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Fast-to-slow shift of muscle fiber-type composition by dietary apple polyphenols in rats : Impact of the low-dose supplementation. / Mizunoya, Wataru; Okamoto, Shinpei; Miyahara, Hideo; Akahoshi, Mariko; Suzuki, Takahiro; Do, Mai Khoi Q.; Ohtsubo, Hideaki; Komiya, Yusuke; Qahar, Mulan; Waga, Toshiaki; Nakazato, Koichi; Ikeuchi, Yoshihide; Anderson, Judy E.; Tatsumi, Ryuichi.

In: Animal Science Journal, Vol. 88, No. 3, 01.03.2017, p. 489-499.

Research output: Contribution to journalArticle

Mizunoya, W, Okamoto, S, Miyahara, H, Akahoshi, M, Suzuki, T, Do, MKQ, Ohtsubo, H, Komiya, Y, Qahar, M, Waga, T, Nakazato, K, Ikeuchi, Y, Anderson, JE & Tatsumi, R 2017, 'Fast-to-slow shift of muscle fiber-type composition by dietary apple polyphenols in rats: Impact of the low-dose supplementation', Animal Science Journal, vol. 88, no. 3, pp. 489-499. https://doi.org/10.1111/asj.12655
Mizunoya, Wataru ; Okamoto, Shinpei ; Miyahara, Hideo ; Akahoshi, Mariko ; Suzuki, Takahiro ; Do, Mai Khoi Q. ; Ohtsubo, Hideaki ; Komiya, Yusuke ; Qahar, Mulan ; Waga, Toshiaki ; Nakazato, Koichi ; Ikeuchi, Yoshihide ; Anderson, Judy E. ; Tatsumi, Ryuichi. / Fast-to-slow shift of muscle fiber-type composition by dietary apple polyphenols in rats : Impact of the low-dose supplementation. In: Animal Science Journal. 2017 ; Vol. 88, No. 3. pp. 489-499.
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