Fatty Acid-Mediated Stromal Reprogramming of Pancreatic Stellate Cells Induces Inflammation and Fibrosis That Fuels Pancreatic Cancer

Tomoki Hata, Koichi Kawamoto, Hidetoshi Eguchi, Yoshihiro Kamada, Shinji Takamatsu, Tomohiro Maekawa, Satoshi Nagaoka, Daisaku Yamada, Yoshifumi Iwagami, Tadafumi Asaoka, Takehiro Noda, Hiroshi Wada, Kunihito Gotoh, Atsushi Masamune, Eiji Miyoshi, Masaki Mori, Yuichiro Doki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives Pancreatic ductal adenocarcinoma is one of the deadliest diseases worldwide. Fatty acids (FAs) have properties that affect both cancer cells and tumor environment. We assessed the effects of FAs on malignant characteristics in a pancreatic cancer and pancreatic stellate cell (PSC) coculture model. This study aimed to clarify the FA signature of PSC-derived inflammation and fibrosis in vitro and in a clinicopathological analysis. Methods The in vitro model involved coculture of the human pancreatic cancer cell lines PANC-1 and MIA PaCa-2 with human PSCs. Clinical histological samples were analyzed to characterize the surgical margins of samples from patients who received distal pancreatectomies. Results The pancreatic cancer cells took up lipids from the culture media. Saturated and unsaturated FAs were required to induce inflammatory responses in human PSCs, and the cocultures showed fibrotic changes. Clinical samples from pancreatic ductal adenocarcinoma patients had more fatty and fibrotic changes in the normal tissue in the surgical margins than samples from noncancer patients. Conclusions Inflammation and fibrosis levels were increased in pancreatic cancer specimens, supporting the in vitro observations and suggesting that PSCs contribute to pancreatic carcinogenesis. Pancreatic stellate cells thus represent a potential therapeutic target for suppressing stromal changes in pancreatic cancer.

Original languageEnglish
Pages (from-to)1259-1266
Number of pages8
JournalPancreas
Volume46
Issue number10
DOIs
Publication statusPublished - Nov 1 2017
Externally publishedYes

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Pancreatic Stellate Cells
Pancreatic Neoplasms
Fibrosis
Fatty Acids
Inflammation
Coculture Techniques
Adenocarcinoma
Pancreatectomy
Unsaturated Fatty Acids
Culture Media
Neoplasms
Carcinogenesis
Lipids
Cell Line
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Fatty Acid-Mediated Stromal Reprogramming of Pancreatic Stellate Cells Induces Inflammation and Fibrosis That Fuels Pancreatic Cancer. / Hata, Tomoki; Kawamoto, Koichi; Eguchi, Hidetoshi; Kamada, Yoshihiro; Takamatsu, Shinji; Maekawa, Tomohiro; Nagaoka, Satoshi; Yamada, Daisaku; Iwagami, Yoshifumi; Asaoka, Tadafumi; Noda, Takehiro; Wada, Hiroshi; Gotoh, Kunihito; Masamune, Atsushi; Miyoshi, Eiji; Mori, Masaki; Doki, Yuichiro.

In: Pancreas, Vol. 46, No. 10, 01.11.2017, p. 1259-1266.

Research output: Contribution to journalArticle

Hata, T, Kawamoto, K, Eguchi, H, Kamada, Y, Takamatsu, S, Maekawa, T, Nagaoka, S, Yamada, D, Iwagami, Y, Asaoka, T, Noda, T, Wada, H, Gotoh, K, Masamune, A, Miyoshi, E, Mori, M & Doki, Y 2017, 'Fatty Acid-Mediated Stromal Reprogramming of Pancreatic Stellate Cells Induces Inflammation and Fibrosis That Fuels Pancreatic Cancer', Pancreas, vol. 46, no. 10, pp. 1259-1266. https://doi.org/10.1097/MPA.0000000000000943
Hata, Tomoki ; Kawamoto, Koichi ; Eguchi, Hidetoshi ; Kamada, Yoshihiro ; Takamatsu, Shinji ; Maekawa, Tomohiro ; Nagaoka, Satoshi ; Yamada, Daisaku ; Iwagami, Yoshifumi ; Asaoka, Tadafumi ; Noda, Takehiro ; Wada, Hiroshi ; Gotoh, Kunihito ; Masamune, Atsushi ; Miyoshi, Eiji ; Mori, Masaki ; Doki, Yuichiro. / Fatty Acid-Mediated Stromal Reprogramming of Pancreatic Stellate Cells Induces Inflammation and Fibrosis That Fuels Pancreatic Cancer. In: Pancreas. 2017 ; Vol. 46, No. 10. pp. 1259-1266.
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abstract = "Objectives Pancreatic ductal adenocarcinoma is one of the deadliest diseases worldwide. Fatty acids (FAs) have properties that affect both cancer cells and tumor environment. We assessed the effects of FAs on malignant characteristics in a pancreatic cancer and pancreatic stellate cell (PSC) coculture model. This study aimed to clarify the FA signature of PSC-derived inflammation and fibrosis in vitro and in a clinicopathological analysis. Methods The in vitro model involved coculture of the human pancreatic cancer cell lines PANC-1 and MIA PaCa-2 with human PSCs. Clinical histological samples were analyzed to characterize the surgical margins of samples from patients who received distal pancreatectomies. Results The pancreatic cancer cells took up lipids from the culture media. Saturated and unsaturated FAs were required to induce inflammatory responses in human PSCs, and the cocultures showed fibrotic changes. Clinical samples from pancreatic ductal adenocarcinoma patients had more fatty and fibrotic changes in the normal tissue in the surgical margins than samples from noncancer patients. Conclusions Inflammation and fibrosis levels were increased in pancreatic cancer specimens, supporting the in vitro observations and suggesting that PSCs contribute to pancreatic carcinogenesis. Pancreatic stellate cells thus represent a potential therapeutic target for suppressing stromal changes in pancreatic cancer.",
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AU - Eguchi, Hidetoshi

AU - Kamada, Yoshihiro

AU - Takamatsu, Shinji

AU - Maekawa, Tomohiro

AU - Nagaoka, Satoshi

AU - Yamada, Daisaku

AU - Iwagami, Yoshifumi

AU - Asaoka, Tadafumi

AU - Noda, Takehiro

AU - Wada, Hiroshi

AU - Gotoh, Kunihito

AU - Masamune, Atsushi

AU - Miyoshi, Eiji

AU - Mori, Masaki

AU - Doki, Yuichiro

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N2 - Objectives Pancreatic ductal adenocarcinoma is one of the deadliest diseases worldwide. Fatty acids (FAs) have properties that affect both cancer cells and tumor environment. We assessed the effects of FAs on malignant characteristics in a pancreatic cancer and pancreatic stellate cell (PSC) coculture model. This study aimed to clarify the FA signature of PSC-derived inflammation and fibrosis in vitro and in a clinicopathological analysis. Methods The in vitro model involved coculture of the human pancreatic cancer cell lines PANC-1 and MIA PaCa-2 with human PSCs. Clinical histological samples were analyzed to characterize the surgical margins of samples from patients who received distal pancreatectomies. Results The pancreatic cancer cells took up lipids from the culture media. Saturated and unsaturated FAs were required to induce inflammatory responses in human PSCs, and the cocultures showed fibrotic changes. Clinical samples from pancreatic ductal adenocarcinoma patients had more fatty and fibrotic changes in the normal tissue in the surgical margins than samples from noncancer patients. Conclusions Inflammation and fibrosis levels were increased in pancreatic cancer specimens, supporting the in vitro observations and suggesting that PSCs contribute to pancreatic carcinogenesis. Pancreatic stellate cells thus represent a potential therapeutic target for suppressing stromal changes in pancreatic cancer.

AB - Objectives Pancreatic ductal adenocarcinoma is one of the deadliest diseases worldwide. Fatty acids (FAs) have properties that affect both cancer cells and tumor environment. We assessed the effects of FAs on malignant characteristics in a pancreatic cancer and pancreatic stellate cell (PSC) coculture model. This study aimed to clarify the FA signature of PSC-derived inflammation and fibrosis in vitro and in a clinicopathological analysis. Methods The in vitro model involved coculture of the human pancreatic cancer cell lines PANC-1 and MIA PaCa-2 with human PSCs. Clinical histological samples were analyzed to characterize the surgical margins of samples from patients who received distal pancreatectomies. Results The pancreatic cancer cells took up lipids from the culture media. Saturated and unsaturated FAs were required to induce inflammatory responses in human PSCs, and the cocultures showed fibrotic changes. Clinical samples from pancreatic ductal adenocarcinoma patients had more fatty and fibrotic changes in the normal tissue in the surgical margins than samples from noncancer patients. Conclusions Inflammation and fibrosis levels were increased in pancreatic cancer specimens, supporting the in vitro observations and suggesting that PSCs contribute to pancreatic carcinogenesis. Pancreatic stellate cells thus represent a potential therapeutic target for suppressing stromal changes in pancreatic cancer.

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