Fc receptor γ-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils

Shigeaki Hida, Shou Yamasaki, Yuzuru Sakamoto, Masaya Takamoto, Kazushige Obata, Toshiyuki Takai, Hajime Karasuyama, Kazuo Sugane, Takashi Saito, Shinsuke Taki

    Research output: Contribution to journalArticle

    65 Citations (Scopus)

    Abstract

    The Fc receptor common γ-chain (FcRγ) is a widely expressed adaptor bearing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. We show here that basophils lacking FcRγ developed normally and proliferated efficiently in response to interleukin 3 (IL-3) but were very impaired in IL-3-induced production of IL-4 and in supporting T helper type 2 differentiation. Through its transmembrane portion, FcRγ associated constitutively with the common β-chain of the IL-3 receptor and signaled by recruiting the kinase Syk. Retrovirus-mediated complementation demonstrated the essential function of the ITAM of FcRγ in IL-3 signal transduction. Our results identify a previously unknown mechanism whereby FcRγ functions to 'route' selective cytokine-triggered signals into the ITAM-mediated IL-4 production pathway.

    Original languageEnglish
    Pages (from-to)214-222
    Number of pages9
    JournalNature Immunology
    Volume10
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 2009

    Fingerprint

    Interleukin-3 Receptors
    Immunoreceptor Tyrosine-Based Activation Motif
    Basophils
    Fc Receptors
    Interleukin-3
    Interleukin-4
    Retroviridae
    Signal Transduction
    Phosphotransferases
    Cytokines

    All Science Journal Classification (ASJC) codes

    • Immunology

    Cite this

    Fc receptor γ-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils. / Hida, Shigeaki; Yamasaki, Shou; Sakamoto, Yuzuru; Takamoto, Masaya; Obata, Kazushige; Takai, Toshiyuki; Karasuyama, Hajime; Sugane, Kazuo; Saito, Takashi; Taki, Shinsuke.

    In: Nature Immunology, Vol. 10, No. 2, 01.01.2009, p. 214-222.

    Research output: Contribution to journalArticle

    Hida, S, Yamasaki, S, Sakamoto, Y, Takamoto, M, Obata, K, Takai, T, Karasuyama, H, Sugane, K, Saito, T & Taki, S 2009, 'Fc receptor γ-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils', Nature Immunology, vol. 10, no. 2, pp. 214-222. https://doi.org/10.1038/ni.1686
    Hida, Shigeaki ; Yamasaki, Shou ; Sakamoto, Yuzuru ; Takamoto, Masaya ; Obata, Kazushige ; Takai, Toshiyuki ; Karasuyama, Hajime ; Sugane, Kazuo ; Saito, Takashi ; Taki, Shinsuke. / Fc receptor γ-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils. In: Nature Immunology. 2009 ; Vol. 10, No. 2. pp. 214-222.
    @article{9c333d2baaeb481691188ab65c476618,
    title = "Fc receptor γ-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils",
    abstract = "The Fc receptor common γ-chain (FcRγ) is a widely expressed adaptor bearing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. We show here that basophils lacking FcRγ developed normally and proliferated efficiently in response to interleukin 3 (IL-3) but were very impaired in IL-3-induced production of IL-4 and in supporting T helper type 2 differentiation. Through its transmembrane portion, FcRγ associated constitutively with the common β-chain of the IL-3 receptor and signaled by recruiting the kinase Syk. Retrovirus-mediated complementation demonstrated the essential function of the ITAM of FcRγ in IL-3 signal transduction. Our results identify a previously unknown mechanism whereby FcRγ functions to 'route' selective cytokine-triggered signals into the ITAM-mediated IL-4 production pathway.",
    author = "Shigeaki Hida and Shou Yamasaki and Yuzuru Sakamoto and Masaya Takamoto and Kazushige Obata and Toshiyuki Takai and Hajime Karasuyama and Kazuo Sugane and Takashi Saito and Shinsuke Taki",
    year = "2009",
    month = "1",
    day = "1",
    doi = "10.1038/ni.1686",
    language = "English",
    volume = "10",
    pages = "214--222",
    journal = "Nature Immunology",
    issn = "1529-2908",
    publisher = "Nature Publishing Group",
    number = "2",

    }

    TY - JOUR

    T1 - Fc receptor γ-chain, a constitutive component of the IL-3 receptor, is required for IL-3-induced IL-4 production in basophils

    AU - Hida, Shigeaki

    AU - Yamasaki, Shou

    AU - Sakamoto, Yuzuru

    AU - Takamoto, Masaya

    AU - Obata, Kazushige

    AU - Takai, Toshiyuki

    AU - Karasuyama, Hajime

    AU - Sugane, Kazuo

    AU - Saito, Takashi

    AU - Taki, Shinsuke

    PY - 2009/1/1

    Y1 - 2009/1/1

    N2 - The Fc receptor common γ-chain (FcRγ) is a widely expressed adaptor bearing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. We show here that basophils lacking FcRγ developed normally and proliferated efficiently in response to interleukin 3 (IL-3) but were very impaired in IL-3-induced production of IL-4 and in supporting T helper type 2 differentiation. Through its transmembrane portion, FcRγ associated constitutively with the common β-chain of the IL-3 receptor and signaled by recruiting the kinase Syk. Retrovirus-mediated complementation demonstrated the essential function of the ITAM of FcRγ in IL-3 signal transduction. Our results identify a previously unknown mechanism whereby FcRγ functions to 'route' selective cytokine-triggered signals into the ITAM-mediated IL-4 production pathway.

    AB - The Fc receptor common γ-chain (FcRγ) is a widely expressed adaptor bearing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. We show here that basophils lacking FcRγ developed normally and proliferated efficiently in response to interleukin 3 (IL-3) but were very impaired in IL-3-induced production of IL-4 and in supporting T helper type 2 differentiation. Through its transmembrane portion, FcRγ associated constitutively with the common β-chain of the IL-3 receptor and signaled by recruiting the kinase Syk. Retrovirus-mediated complementation demonstrated the essential function of the ITAM of FcRγ in IL-3 signal transduction. Our results identify a previously unknown mechanism whereby FcRγ functions to 'route' selective cytokine-triggered signals into the ITAM-mediated IL-4 production pathway.

    UR - http://www.scopus.com/inward/record.url?scp=58549089167&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=58549089167&partnerID=8YFLogxK

    U2 - 10.1038/ni.1686

    DO - 10.1038/ni.1686

    M3 - Article

    VL - 10

    SP - 214

    EP - 222

    JO - Nature Immunology

    JF - Nature Immunology

    SN - 1529-2908

    IS - 2

    ER -