Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression

Mohamed Ali Sayed Mohamed Abdelrahman, Yaotian Xiao, Yukihiro Tashiro, Ying Wang, Takeshi Zendo, Kenji Sakai, Kenji Sonomoto

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25g/L; therefore, initial concentrations of 25g/L glucose and 50g/L xylose were selected. Supplementation with 5g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60g/(L·h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume119
Issue number2
DOIs
Publication statusPublished - Feb 1 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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