TY - JOUR
T1 - Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression
AU - Abdel-Rahman, Mohamed Ali
AU - Xiao, Yaotian
AU - Tashiro, Yukihiro
AU - Wang, Ying
AU - Zendo, Takeshi
AU - Sakai, Kenji
AU - Sonomoto, Kenji
N1 - Funding Information:
This study has been supported by a Grant-in-Aid ( P12088 ) for JSPS Fellows to Mohamed Ali Abdel-Rahman from the Japan Society for the Promotion of Science (JSPS) , Japan. This research was financially supported in part by the Sumitomo Corporation (Japan).
Publisher Copyright:
© 2014 The Society for Biotechnology, Japan.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25g/L; therefore, initial concentrations of 25g/L glucose and 50g/L xylose were selected. Supplementation with 5g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60g/(L·h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.
AB - There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25g/L; therefore, initial concentrations of 25g/L glucose and 50g/L xylose were selected. Supplementation with 5g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60g/(L·h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.
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U2 - 10.1016/j.jbiosc.2014.07.007
DO - 10.1016/j.jbiosc.2014.07.007
M3 - Article
C2 - 25280397
AN - SCOPUS:84922526865
SN - 1389-1723
VL - 119
SP - 153
EP - 158
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 2
ER -