Abstract
There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25g/L; therefore, initial concentrations of 25g/L glucose and 50g/L xylose were selected. Supplementation with 5g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60g/(L·h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.
| Original language | English |
|---|---|
| Pages (from-to) | 153-158 |
| Number of pages | 6 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 119 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2015 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression. / Abdelrahman, Mohamed Ali Sayed Mohamed; Xiao, Yaotian; Tashiro, Yukihiro; Wang, Ying; Zendo, Takeshi; Sakai, Kenji; Sonomoto, Kenji.
In: Journal of Bioscience and Bioengineering, Vol. 119, No. 2, 01.02.2015, p. 153-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression
AU - Abdelrahman, Mohamed Ali Sayed Mohamed
AU - Xiao, Yaotian
AU - Tashiro, Yukihiro
AU - Wang, Ying
AU - Zendo, Takeshi
AU - Sakai, Kenji
AU - Sonomoto, Kenji
PY - 2015/2/1
Y1 - 2015/2/1
N2 - There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25g/L; therefore, initial concentrations of 25g/L glucose and 50g/L xylose were selected. Supplementation with 5g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60g/(L·h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.
AB - There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25g/L; therefore, initial concentrations of 25g/L glucose and 50g/L xylose were selected. Supplementation with 5g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60g/(L·h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.
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UR - http://www.scopus.com/inward/citedby.url?scp=84922526865&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2014.07.007
DO - 10.1016/j.jbiosc.2014.07.007
M3 - Article
C2 - 25280397
AN - SCOPUS:84922526865
VL - 119
SP - 153
EP - 158
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 2
ER -