Feedback regulation of β-thujaplicin production and formation of its methyl ether in a suspension culture of Cupressus lusitanica

Junko Yamada, Koki Fujita, Kokki Sakai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Suspension cell cultures of Cupressus lusitanica produce β-thujaplicin, a tropolone found mostly in Cupressaceae heartwood. The factors controlling β-thujaplicin accumulation in this cell culture system were investigated. Initial cell density of the cultures did not affect β-thujaplicin levels, though initial addition of β-thujaplicin suppressed its de novo production. When β-thujaplicin accumulation reached a certain level (ca. 40 mg/l) in the medium, the cultures seemed to cease β-thujaplicin production. However, β-thujaplicin productivity was restored when the β-thujaplicin-containing medium was exchanged for fresh medium; the formation of 2-methoxy-6-(methylethyl)cyclohepta-2,4,6-trien-1-one, an isomer of methylated β-thujaplicin, in medium was also observed. These results suggest that β-thujaplicin synthesis was regulated by product feedback mechanism in this cell line, and that excess accumulation of β-thujaplicin is relieved by conversion of β-thujaplicin to its methyl ether.

Original languageEnglish
Pages (from-to)447-450
Number of pages4
JournalPhytochemistry
Volume60
Issue number5
DOIs
Publication statusPublished - Jul 1 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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