Feedback regulation of β-thujaplicin production and formation of its methyl ether in a suspension culture of Cupressus lusitanica

Junko Yamada, Koki Fujita, Kokki Sakai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Suspension cell cultures of Cupressus lusitanica produce β-thujaplicin, a tropolone found mostly in Cupressaceae heartwood. The factors controlling β-thujaplicin accumulation in this cell culture system were investigated. Initial cell density of the cultures did not affect β-thujaplicin levels, though initial addition of β-thujaplicin suppressed its de novo production. When β-thujaplicin accumulation reached a certain level (ca. 40 mg/l) in the medium, the cultures seemed to cease β-thujaplicin production. However, β-thujaplicin productivity was restored when the β-thujaplicin-containing medium was exchanged for fresh medium; the formation of 2-methoxy-6-(methylethyl)cyclohepta-2,4,6-trien-1-one, an isomer of methylated β-thujaplicin, in medium was also observed. These results suggest that β-thujaplicin synthesis was regulated by product feedback mechanism in this cell line, and that excess accumulation of β-thujaplicin is relieved by conversion of β-thujaplicin to its methyl ether.

Original languageEnglish
Pages (from-to)447-450
Number of pages4
JournalPhytochemistry
Volume60
Issue number5
DOIs
Publication statusPublished - Jul 1 2002

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tropolone
Cupressus lusitanica
Cupressus
Methyl Ethers
Cupressaceae
heartwood
Cell culture
cell suspension culture
isomers
ethers
Suspensions
cell culture
Cell Culture Techniques
Tropolone
culture media
cell lines
Trientine
Feedback
synthesis
Isomers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

Cite this

Feedback regulation of β-thujaplicin production and formation of its methyl ether in a suspension culture of Cupressus lusitanica. / Yamada, Junko; Fujita, Koki; Sakai, Kokki.

In: Phytochemistry, Vol. 60, No. 5, 01.07.2002, p. 447-450.

Research output: Contribution to journalArticle

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