Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation

Xiao Shan Yue, Masako Fujishiro, Chieko Nishioka, Takashi Arai, Eiki Takahashi, Jian Sheng Gong, Toshihiro Akaike, Yoshihiro Ito

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cells, as well as their undifferentiated state. Furthermore, the chemically fixed MEF feeders were reused several times without affecting their functions. These results indicate that chemical fixation can be applied to modify biological feeders chemically, without losing their original functions. Chemically fixed MEF feeders will be applicable to other stem cell cultures as a reusable extracellular matrix candidate that can be preserved on a long-term basis.

Original languageEnglish
Article numbere32707
JournalPloS one
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 2 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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