Ferrimicrobium acidiphilum gen. nov., sp. nov. and Ferrithrix thermotolerans gen. nov., sp. nov. Heterotrophic, iron-oxidizing, extremely acidophilic actinobacteria

D. Barrie Johnson, Paula Bacelar-Nicolau, Naoko Okibe, Angharad Thomas, Kevin B. Hallberg

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Two novel extremely acidophilic, iron-oxidizing actinobacteria were isolated, one from a mine site in North Wales, UK (isolate T23T), and the other from a geothermal site in Yellowstone National Park, Wyoming, USA (Y005T). These new actinobacteria belong to the subclass Acidimicrobidae, and in contrast to the only other classified member of the subclass (Acidimicrobium ferrooxidans), both isolates were obligate heterotrophs. The mine site isolate was mesophilic and grew as small rods, while the Yellowstone isolate was a moderate thermophile and grew as long filaments, forming macroscopic flocs in liquid media. Both isolates accelerated the oxidative dissolution of pyrite in yeast extract-amended cultures, but neither was able to oxidize reduced forms of sulfur. Ferrous iron oxidation enhanced growth yields of the novel mesophilic actinobacterium T23T, though this was not confirmed for the Yellowstone isolate. Both isolates catalysed the dissimilatory reduction of ferric iron, using glycerol as electron donor, in oxygen-free medium. Based on comparative analyses of base compositions of their chromosomal DNA and of their 16S rRNA gene sequences, the isolates are both distinct from each other and from Acidimicrobium ferrooxidans, and are representatives of two novel genera. The names Ferrimicrobium acidiphilum gen. nov., sp. nov. and Ferrithrix thermotolerans gen. nov., sp. nov. are proposed for the mesophilic and moderately thermophilic isolates, respectively, with the respective type strains T23T (=DSM 19497T=ATCC BAA-1647T) and Y005T (=DSM 19514T=ATCC BAA-1645T).

Original languageEnglish
Pages (from-to)1082-1089
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume59
Issue number5
DOIs
Publication statusPublished - Nov 10 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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