Finding a novel beta-(1→3)-D-Glucan/polynucleotide complex and its applications for an immunocyte-targeting delivery

Noriko Miyamoto, Shinichi Mochizuki, Seiji Shinkai, Kazuo Sakurai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Schizophyllan (SPG) is a natural beta-(1→3)-D-glucan existing as a triple helix in water and as a single chain in alkaline solutions, respectively. When homo-polynucleotides such as poly(dA) are added to SPG alkaline solution and subsequently pH is adjusted to be neutral, the single chain of SPG forms a stoichiometric complex with the certain polynucleotides. The complex can protect the bound DNA against nuclease-mediated hydrolysis or non-specific binding to serum proteins. Furthermore, recent immunology revealed that activated antigen presenting cells including dendritic immune cells express a receptor called Dectin-1. We have demonstrated that Dectin-1 recognizes SPG/oligonucleotide (ODN) complexes, and the complex is eventually ingested by the cells. This finding suggests that SPG/ODN complex can be specifically ingested by activated immunocytes and the bound ODN can exert its effect after ingestion. The present review will show our recent in-vivo and vitro studies to prove this idea and provide a new strategy to specifically transport functional ODNs including antisense (AS)-ODN, CpG-ODN, and siRNA to antigen presenting cells (APCs) to cure the diseases due to disorientation of immune.

Original languageEnglish
Title of host publicationBeta-Glucans
Subtitle of host publicationApplications, Effects and Research
PublisherNova Science Publishers, Inc.
Pages1-33
Number of pages33
ISBN (Electronic)9781536106558
ISBN (Print)9781536106282
Publication statusPublished - Jan 1 2017

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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    Miyamoto, N., Mochizuki, S., Shinkai, S., & Sakurai, K. (2017). Finding a novel beta-(1→3)-D-Glucan/polynucleotide complex and its applications for an immunocyte-targeting delivery. In Beta-Glucans: Applications, Effects and Research (pp. 1-33). Nova Science Publishers, Inc..