Finding the relationships between a strain of Bacillus thuringiensis, tannic acid, and the mid-gut proteases of Spodoptera exigua

Na Young Jin, You Kyoung Lee, Bo Ram Lee, Yu Seop Kim, Jun Hack Jun, Hee Ji Kim, Young Nam Youn, Chisa Yasunaga-Aoki, Yong Man Yu

Research output: Contribution to journalArticle

Abstract

A new strain of Bacillus thuringiensis exhibiting activity in Spodoptera exigua was isolated and identified. Synergistic effects appeared when it was mixed with tannic acid, which is a protease inhibitor that enhances insecticidal activities. Substrate reactions with different types of proteases were analyzed, and according to the results, trypsin showed proteolytic activity levels of 91.4% and 89.4% on BApNA and BPVApNA substrates, respectively. When trypsin was treated with tannic acid, proteolytic activity levels of 62.2% and 54.5% appeared on BApNA and BPVApNA substrates, respectively, and tannic acid suppressed the trypsin-substrate reactions by 29.2% and 34.9%, respectively. Colorimetric activity assays of trypsin identified slightly lower activity levels in larvae that ate a combination of B. thuringiensis KB100 strain and 40 mM of tannic acid, when compared to larvae that died after eating the positive control, control, or the B. thuringiensis subsp. kurstaki KB100. The results of trypsin gene expression level analyses conducted using the qRT-PCR analyses indicated that trypsin 1 gene expression levels were lower when 40 mM of tannic acid was added to the B. thuringiensis KB100 strain, compared to the B. thuringiensis KB100 strain alone. Trypsin 2 gene expression levels were also lower when 40 mM of tannic acid was added to the B. thuringiensis KB100 strain. The results of immunofluorescence antibody experiments indicated that the addition of 40 mM of tannic acid to the B. thuringiensis KB100 strain, during decomposition by the mid-gut proteases of 5. exigua, led to differences in expression levels and image analyses.

Original languageEnglish
Pages (from-to)319-329
Number of pages11
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume61
Issue number2
Publication statusPublished - Sep 1 2016

Fingerprint

Spodoptera
Bacillus thuringiensis
Spodoptera exigua
Tannins
midgut
Trypsin
trypsin
tannins
Peptide Hydrolases
proteinases
Gene Expression
gene expression
Larva
Bacillus thuringiensis subsp. kurstaki
larvae
proteinase inhibitors
Protease Inhibitors
fluorescent antibody technique
Fluorescent Antibody Technique
Eating

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Finding the relationships between a strain of Bacillus thuringiensis, tannic acid, and the mid-gut proteases of Spodoptera exigua. / Jin, Na Young; Lee, You Kyoung; Lee, Bo Ram; Kim, Yu Seop; Jun, Jun Hack; Kim, Hee Ji; Youn, Young Nam; Yasunaga-Aoki, Chisa; Yu, Yong Man.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 61, No. 2, 01.09.2016, p. 319-329.

Research output: Contribution to journalArticle

Jin, Na Young ; Lee, You Kyoung ; Lee, Bo Ram ; Kim, Yu Seop ; Jun, Jun Hack ; Kim, Hee Ji ; Youn, Young Nam ; Yasunaga-Aoki, Chisa ; Yu, Yong Man. / Finding the relationships between a strain of Bacillus thuringiensis, tannic acid, and the mid-gut proteases of Spodoptera exigua. In: Journal of the Faculty of Agriculture, Kyushu University. 2016 ; Vol. 61, No. 2. pp. 319-329.
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