Fine fractionation and purification of the fulvic acid fraction using adsorption and precipitation procedures

Syuntaro Hiradate, Takuya Yonezawa, Hiroshi Takesako

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A new scheme for fine fractionating and purifying a crude fulvic acid fraction was proposed. The fractionation procedure includes an adsorption process of the fulvic acid fraction on a hydrophobic resin (e.g. XAD-8 and DAX-8) and a sequence of successive elution processes. This procedure yields a non-adsorbed fraction (FA-1), a 0.1 mol L-1 HCl eluted fraction (FA-2), a distilled water eluted fraction (FA-3) and a 0.1 mol L-1 NaOH eluted fraction (FA-4). To remove salts and water, the fractionated organic molecules were co-precipitated with Al hydroxides at pH 5 and collected by centrifugation. Recoveries of carbon from the crude fulvic acid fraction of an Andosol were 42, 20, 19 and 14% for FA-1, FA-2, FA-3 and FA-4, respectively, and 96% of carbon was recovered in total. Optical properties and 13C nuclear magnetic resonance spectra indicated that the chemical properties of FA-1 were similar to those of FA-2 and were hydrophilic, while the chemical properties of FA-3 were very close to those of FA-4 and were hydrophobic (estimated hydrophobicity: humic acid >> FA-4 ≥ FA-3 >> FA-2 ≥ FA-1). A fulvic acid that can be isolated using the International Humic Substances Society method would be, in practice, a mixture of hydrophilic fulvic acid (FA-2) and hydrophobic fulvic acid (FA-3 + FA-4). The method developed in the present study will provide more detailed, quantitative and complete information of the fulvic acid fraction of soils.

Original languageEnglish
Pages (from-to)413-419
Number of pages7
JournalSoil Science and Plant Nutrition
Volume53
Issue number4
DOIs
Publication statusPublished - Aug 1 2007
Externally publishedYes

Fingerprint

fulvic acids
fulvic acid
purification
fractionation
adsorption
chemical property
physicochemical properties
soil separates
Andosol
hydroxides
carbon
optical properties
humic substances
hydrophobicity
humic acids
humic substance
optical property
humic acid
centrifugation
resins

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Agronomy and Crop Science

Cite this

Fine fractionation and purification of the fulvic acid fraction using adsorption and precipitation procedures. / Hiradate, Syuntaro; Yonezawa, Takuya; Takesako, Hiroshi.

In: Soil Science and Plant Nutrition, Vol. 53, No. 4, 01.08.2007, p. 413-419.

Research output: Contribution to journalArticle

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