First cultivation and ecological investigation of a bacterium affiliated with the candidate phylum OP5 from hot springs

Koji Mori, Michinari Sunamura, Katsunori Yanagawa, Junichiro Ishibashi, Youko Miyoshi, Takao Iino, Ken Ichiro Suzuki, Tetsuro Urabe

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The phylogenetic group termed OP5 was originally discovered in the Yellowstone National Park hot spring and proposed as an uncultured phylum; the group was afterwards analyzed by applying culture-independent approaches. Recently, a novel thermophilic chemoheterotrophic filamentous bacterium was obtained from a hot spring in Japan that was enriched through various isolation procedures. Phylogenetic analyses of the isolate have revealed that it is closely related to the OP5 phylum that has mainly been constructed with the environmental clones retrieved from thermophilic and mesophilic anaerobic environments. It appears that the lineage is independent at the phylum level in the domain Bacteria. Therefore, we designed a primer set for the 16S rRNA gene to specifically target the OP5 phylum and performed quantitative field analysis by using the real-time PCR method. Thus, the 16S rRNA gene of the OP5 phylum was detected in some hot-spring samples with the relative abundance ranging from 0.2% to 1.4% of the prokaryotic organisms detected. The physiology of the above-mentioned isolate and the related environmental clones indicated that they are scavengers contributing to the sulfur cycle in nature.

Original languageEnglish
Pages (from-to)6223-6229
Number of pages7
JournalApplied and environmental microbiology
Volume74
Issue number20
DOIs
Publication statusPublished - Oct 1 2008

Fingerprint

Hot Springs
hot springs
thermal spring
Bacteria
rRNA Genes
bacterium
clone
bacteria
Clone Cells
ribosomal RNA
clones
phylogenetics
sulfur cycle
gene
phylogeny
scavenger
Sulfur
physiology
Real-Time Polymerase Chain Reaction
relative abundance

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

First cultivation and ecological investigation of a bacterium affiliated with the candidate phylum OP5 from hot springs. / Mori, Koji; Sunamura, Michinari; Yanagawa, Katsunori; Ishibashi, Junichiro; Miyoshi, Youko; Iino, Takao; Suzuki, Ken Ichiro; Urabe, Tetsuro.

In: Applied and environmental microbiology, Vol. 74, No. 20, 01.10.2008, p. 6223-6229.

Research output: Contribution to journalArticle

Mori, Koji ; Sunamura, Michinari ; Yanagawa, Katsunori ; Ishibashi, Junichiro ; Miyoshi, Youko ; Iino, Takao ; Suzuki, Ken Ichiro ; Urabe, Tetsuro. / First cultivation and ecological investigation of a bacterium affiliated with the candidate phylum OP5 from hot springs. In: Applied and environmental microbiology. 2008 ; Vol. 74, No. 20. pp. 6223-6229.
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AU - Miyoshi, Youko

AU - Iino, Takao

AU - Suzuki, Ken Ichiro

AU - Urabe, Tetsuro

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