Fixation and accumulation of thermotolerant catalytic competence of a pair of ligase ribozymes through complex formation and cross ligation

Nana Isomoto, Yuri Maeda, Takahiro Tanaka, Hiroyuki Furuta, Yoshiya Ikawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the early stages of the hypothetical RNA world, some primitive RNA catalysts (ribozymes) may have emerged through self-assembly of short RNA oligomers. Although they may be unstable against temperature fluctuations and other environmental changes, ligase ribozymes (ribozymes with RNA strand-joining activity) may resolve structural instability of self-assembling RNAs by converting them to the corresponding unimolecular formats. To investigate this possibility, we constructed a model system using a cross-ligation system composed of a pair of self-assembling ligase ribozymes. Their abilities to act as catalysts, substrates, and a cross-ligation system were analyzed with or without thermal pretreatment before the reactions. A pair of self-assembling ligase ribozymes, each of which can form multiple conformations, demonstrated that thermotolerance was acquired and accumulated through complex-formation that stabilized the active forms of the bimolecular ribozymes and also cross-ligation that produced the unimolecular ribozymes.

Original languageEnglish
Pages (from-to)48-58
Number of pages11
JournalJournal of Molecular Evolution
Volume76
Issue number1-2
DOIs
Publication statusPublished - Jan 4 2013

Fingerprint

Catalytic RNA
Ligases
ligases
Mental Competency
fixation
Ligation
RNA
catalysts
catalyst
heat tolerance
Aptitude
environmental change
pretreatment
complex formation
heat
substrate
Hot Temperature
Temperature
temperature

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Fixation and accumulation of thermotolerant catalytic competence of a pair of ligase ribozymes through complex formation and cross ligation. / Isomoto, Nana; Maeda, Yuri; Tanaka, Takahiro; Furuta, Hiroyuki; Ikawa, Yoshiya.

In: Journal of Molecular Evolution, Vol. 76, No. 1-2, 04.01.2013, p. 48-58.

Research output: Contribution to journalArticle

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