FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Hiroshi Nonaka, Sho hei Fujishima, Shohei Uchinomiya, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

Original languageEnglish
Pages (from-to)6696-6699
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number23
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

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Labeling
Proteins
FLAG peptide

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction. / Nonaka, Hiroshi; Fujishima, Sho hei; Uchinomiya, Shohei; Ojida, Akio; Hamachi, Itaru.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 19, No. 23, 01.12.2009, p. 6696-6699.

Research output: Contribution to journalArticle

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