Flavones suppress the expression of the high-affinity IgE receptor FCεRI in human basophilic KU812 cells

Satomi Yano, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We examined the effect of flavones on the expression of the high-affinity IgE receptor FcεRI, which plays a central role in the IgE-mediated allergic response. Flow cytometric analysis showed that the flavones chrysin and apigenin were able to reduce the cell surface expression of FcεRI in human basophilic KU812 cells. Immunoblot analysis revealed that the total cellular expression of the FcεRI α and γ chains decreased upon treatment with chrysin and apigenin. Moreover, the level of mRNA expression of the FcεRI α and γ chains also decreased when the cells were cultured with the two flavones. Previously, we demonstrated that the reduction of extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation was involved in the downregulation of FcεRI expression. The two flavones were shown to reduce the level of ERK1/2 phosphorylation. These results suggested that chrysin and apigenin have the ability to downregulate FcεRI expression and this suppressive effect may be due to the reduction of ERK1/2 phosphorylation.

Original languageEnglish
Pages (from-to)1812-1817
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number5
DOIs
Publication statusPublished - Mar 9 2005

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Flavones
IgE Receptors
flavones
Apigenin
Phosphorylation
Mitogen-Activated Protein Kinase 3
apigenin
Mitogen-Activated Protein Kinase 1
mitogen-activated protein kinase
phosphorylation
receptors
Down-Regulation
cells
Immunoglobulin E
Cultured Cells
Messenger RNA
chrysin

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Flavones suppress the expression of the high-affinity IgE receptor FCεRI in human basophilic KU812 cells. / Yano, Satomi; Tachibana, Hirofumi; Yamada, Koji.

In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 5, 09.03.2005, p. 1812-1817.

Research output: Contribution to journalArticle

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