Flocculation characteristics of an isolated mutant flocculent Saccharomyces cerevisiae strain and its application for fuel ethanol production from kitchen refuse

Kedong Ma, Minato Wakisaka, Kenji Sakai, Yoshihito Shirai

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A stable mutant flocculent yeast strain of Saccharomyces cerevisiae KRM-1 was isolated during repeated-batch ethanol fermentation using kitchen refuse as the medium. The mechanism of flocculation and interaction with the medium was investigated. According to sugar inhibition assay, it was found that the mutant flocculent strain was a NewFlo phenotype. Flocculation was completely inhibited by protease, proteinase K and partially reduced by treatments with carbohydrate-hydrolyzing enzymes. Flocculation ability showed no difference for pH 3.0-6.0. Furthermore, the mutant flocculent yeast provided repeated-batch cultivations employing cell recycles by flocculation over 10 rounds of cultivation for the production of ethanol from kitchen refuse medium, resulting in relatively high productivity averaging 8.25 g/L/h over 10 batches and with a maximal of 10.08 g/L/h in the final batch. Cell recycle by flocculation was fast and convenient, and could therefore be applicable for industrial-scale ethanol production.

Original languageEnglish
Pages (from-to)2289-2292
Number of pages4
JournalBioresource Technology
Volume100
Issue number7
DOIs
Publication statusPublished - Apr 1 2009

Fingerprint

Ethanol fuels
Kitchens
Flocculation
refuse
flocculation
Yeast
ethanol
Ethanol
yeast
Endopeptidase K
Carbohydrates
Sugars
Fermentation
fermentation
phenotype
carbohydrate
Assays
sugar
Peptide Hydrolases
Enzymes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Flocculation characteristics of an isolated mutant flocculent Saccharomyces cerevisiae strain and its application for fuel ethanol production from kitchen refuse. / Ma, Kedong; Wakisaka, Minato; Sakai, Kenji; Shirai, Yoshihito.

In: Bioresource Technology, Vol. 100, No. 7, 01.04.2009, p. 2289-2292.

Research output: Contribution to journalArticle

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