TY - JOUR
T1 - Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain
T2 - Effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments
AU - Ma, Kedong
AU - Wakisaka, Minato
AU - Sakai, Kenji
AU - Shirai, Yoshihito
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2010/2/15
Y1 - 2010/2/15
N2 - The flocculation mechanism of a stable mutant flocculent yeast strain Saccharomyces cerevisiae KRM-1 was quantitatively investigated for potential industrial interest. It was found that the mutant flocculent strain was NewFlo phenotype by means of sugar inhibition test. The flocculation was completely inhibited by treatment with proteinase K, protein-denaturants and carbohydrate modifier. The absence of calcium ions significantly inhibited the flocculation, indicating that Ca2+ was specifically required for flocculation. The flocculation was stable when temperature below 70° C and pH was in the range of 3.0 - 6.0. The flocculation onset of the mutant flocculent strain was in the early stationary growth phase, which coincided with glucose depletion in the batch fermentation for the production of ethanol from kitchen refuse medium. The results are expected to help develop better strategies for the control of mutant flocculent yeast for future large-scale industrial ethanol fermentation.
AB - The flocculation mechanism of a stable mutant flocculent yeast strain Saccharomyces cerevisiae KRM-1 was quantitatively investigated for potential industrial interest. It was found that the mutant flocculent strain was NewFlo phenotype by means of sugar inhibition test. The flocculation was completely inhibited by treatment with proteinase K, protein-denaturants and carbohydrate modifier. The absence of calcium ions significantly inhibited the flocculation, indicating that Ca2+ was specifically required for flocculation. The flocculation was stable when temperature below 70° C and pH was in the range of 3.0 - 6.0. The flocculation onset of the mutant flocculent strain was in the early stationary growth phase, which coincided with glucose depletion in the batch fermentation for the production of ethanol from kitchen refuse medium. The results are expected to help develop better strategies for the control of mutant flocculent yeast for future large-scale industrial ethanol fermentation.
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U2 - 10.5897/ajb09.1541
DO - 10.5897/ajb09.1541
M3 - Article
AN - SCOPUS:77249179802
VL - 9
SP - 1037
EP - 1045
JO - African Journal of Biotechnology
JF - African Journal of Biotechnology
SN - 1684-5315
IS - 7
ER -