Flow injection fluorometric determination of ascorbic acid using perylenebisimide-linked nitroxide

Tomoharu Maki, Nobuaki Soh, Koji Nakano, Toshihiko Imato

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A simple and sensitive flow injection fluorometric method for the determination of ascorbic acid is described. Perylenebisimide-linked nitroxide (PBILN) is used as a fluorescent reagent, which permits the selective determination of ascorbic acid. The fluorescence of the perylenebisimide moiety in PBILN is quenched by the nitroxide moiety, which is linked to the perylenebisimide. When a stream of a solution of ascorbic acid is merged with a stream of PBILN, the ascorbic acid reacts with the nitroxide moiety of PBILN to form hydroxylamine, and the fluorescence properties of the perylenebisimide moiety are recovered. As a result, a peak-shaped fluorescence signal is produced, which can be observed by a fluorescence detector located downstream. Under optimized conditions, a good linear relationship between the concentration of ascorbic acid and peak height in the concentration range from 0.5 to 10 μmol L-1 was found and the detection limit (S/N = 3) was 0.28 μmol L-1. The relative standard deviation for the determination of 4.0 μmol L-1 ascorbic acid samples was 1.0% (n = 5). The proposed method was applied to the determination of ascorbic acid in several soft drink beverages and the analytical results were in good agreement with those obtained using a conventional method.

Original languageEnglish
Pages (from-to)1730-1733
Number of pages4
JournalTalanta
Volume85
Issue number4
DOIs
Publication statusPublished - Sep 30 2011

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Flow injection fluorometric determination of ascorbic acid using perylenebisimide-linked nitroxide'. Together they form a unique fingerprint.

  • Cite this