Fluid shear stress increases interleukin-11 expression in human osteoblast-like cells

Its role in osteoclast induction

Kenji Sakai, Masaaki Mohtai, Jun Ichi Shida, Katsumi Harimaya, Susanna Benvenuti, Maria L. Brandi, Toshio Kukita, Yukihide Iwamoto

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

It is unclear how mechanical stress influences bone cells. Mechanical stress causes fluid shear stress (FSS) in the bone. Osteoblast lineage cells are thought to sense FSS and regulate bone remodeling. We therefore investigated the effects of FSS on human osteoblast-like osteosarcoma cells: SaOS-2 cells in vitro. The conditioned medium of the SaOS-2 cells after 24 h of FSS (24 h-FSS CM) showed such osteoclastic phenotype inductions as significantly increasing the number of tartrate-resistant acid phosphatase (TRAP) positive multinuclear cells in rat bone marrow cells and TRAP-positive cells in human preosteoclastic cells: FLG 29.1 cells. An enzyme-linked immunosorbent assay showed interleukin-11 (IL-11) protein to increase 7-fold in the 24 h-FSS CM. A Northern analysis showed that IL-11 mRNA increased 4- fold in the SaOS-2 cells after 6 h-FSS; however, no IL-6 mRNA expression was detected. Furthermore, the anti-human IL-11 antibody significantly neutralized the osteoclastic phenotype induction of the 24 h-FSS CM. The IL- 11 mRNA up-regulation in SaOS-2 cells by the 6 h-FSS was not inhibited by the anti-human transforming growth factor-β1 antibody, but it was significantly inhibited by indomethacin. An enzymeimmunoassay showed prostaglandin E2 to increase 7-fold in the 1 h-FSS CM. These findings thus suggest that FSS induces osteoblasts to produce IL-11 (mediated by prostaglandins) and thus stimulates bone remodeling.

Original languageEnglish
Pages (from-to)2089-2098
Number of pages10
JournalJournal of Bone and Mineral Research
Volume14
Issue number12
DOIs
Publication statusPublished - Jan 1 1999

Fingerprint

Interleukin-11
Osteoclasts
Osteoblasts
Mechanical Stress
Bone Remodeling
Messenger RNA
Phenotype
Bone and Bones
Antibodies
Transforming Growth Factors
Osteosarcoma
Conditioned Culture Medium
Dinoprostone
Indomethacin
Bone Marrow Cells
Prostaglandins
Interleukin-6
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Cite this

Fluid shear stress increases interleukin-11 expression in human osteoblast-like cells : Its role in osteoclast induction. / Sakai, Kenji; Mohtai, Masaaki; Shida, Jun Ichi; Harimaya, Katsumi; Benvenuti, Susanna; Brandi, Maria L.; Kukita, Toshio; Iwamoto, Yukihide.

In: Journal of Bone and Mineral Research, Vol. 14, No. 12, 01.01.1999, p. 2089-2098.

Research output: Contribution to journalArticle

Sakai, Kenji ; Mohtai, Masaaki ; Shida, Jun Ichi ; Harimaya, Katsumi ; Benvenuti, Susanna ; Brandi, Maria L. ; Kukita, Toshio ; Iwamoto, Yukihide. / Fluid shear stress increases interleukin-11 expression in human osteoblast-like cells : Its role in osteoclast induction. In: Journal of Bone and Mineral Research. 1999 ; Vol. 14, No. 12. pp. 2089-2098.
@article{02bc3cf4c5d54ad695b663a638e6c137,
title = "Fluid shear stress increases interleukin-11 expression in human osteoblast-like cells: Its role in osteoclast induction",
abstract = "It is unclear how mechanical stress influences bone cells. Mechanical stress causes fluid shear stress (FSS) in the bone. Osteoblast lineage cells are thought to sense FSS and regulate bone remodeling. We therefore investigated the effects of FSS on human osteoblast-like osteosarcoma cells: SaOS-2 cells in vitro. The conditioned medium of the SaOS-2 cells after 24 h of FSS (24 h-FSS CM) showed such osteoclastic phenotype inductions as significantly increasing the number of tartrate-resistant acid phosphatase (TRAP) positive multinuclear cells in rat bone marrow cells and TRAP-positive cells in human preosteoclastic cells: FLG 29.1 cells. An enzyme-linked immunosorbent assay showed interleukin-11 (IL-11) protein to increase 7-fold in the 24 h-FSS CM. A Northern analysis showed that IL-11 mRNA increased 4- fold in the SaOS-2 cells after 6 h-FSS; however, no IL-6 mRNA expression was detected. Furthermore, the anti-human IL-11 antibody significantly neutralized the osteoclastic phenotype induction of the 24 h-FSS CM. The IL- 11 mRNA up-regulation in SaOS-2 cells by the 6 h-FSS was not inhibited by the anti-human transforming growth factor-β1 antibody, but it was significantly inhibited by indomethacin. An enzymeimmunoassay showed prostaglandin E2 to increase 7-fold in the 1 h-FSS CM. These findings thus suggest that FSS induces osteoblasts to produce IL-11 (mediated by prostaglandins) and thus stimulates bone remodeling.",
author = "Kenji Sakai and Masaaki Mohtai and Shida, {Jun Ichi} and Katsumi Harimaya and Susanna Benvenuti and Brandi, {Maria L.} and Toshio Kukita and Yukihide Iwamoto",
year = "1999",
month = "1",
day = "1",
doi = "10.1359/jbmr.1999.14.12.2089",
language = "English",
volume = "14",
pages = "2089--2098",
journal = "Journal of Bone and Mineral Research",
issn = "0884-0431",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Fluid shear stress increases interleukin-11 expression in human osteoblast-like cells

T2 - Its role in osteoclast induction

AU - Sakai, Kenji

AU - Mohtai, Masaaki

AU - Shida, Jun Ichi

AU - Harimaya, Katsumi

AU - Benvenuti, Susanna

AU - Brandi, Maria L.

AU - Kukita, Toshio

AU - Iwamoto, Yukihide

PY - 1999/1/1

Y1 - 1999/1/1

N2 - It is unclear how mechanical stress influences bone cells. Mechanical stress causes fluid shear stress (FSS) in the bone. Osteoblast lineage cells are thought to sense FSS and regulate bone remodeling. We therefore investigated the effects of FSS on human osteoblast-like osteosarcoma cells: SaOS-2 cells in vitro. The conditioned medium of the SaOS-2 cells after 24 h of FSS (24 h-FSS CM) showed such osteoclastic phenotype inductions as significantly increasing the number of tartrate-resistant acid phosphatase (TRAP) positive multinuclear cells in rat bone marrow cells and TRAP-positive cells in human preosteoclastic cells: FLG 29.1 cells. An enzyme-linked immunosorbent assay showed interleukin-11 (IL-11) protein to increase 7-fold in the 24 h-FSS CM. A Northern analysis showed that IL-11 mRNA increased 4- fold in the SaOS-2 cells after 6 h-FSS; however, no IL-6 mRNA expression was detected. Furthermore, the anti-human IL-11 antibody significantly neutralized the osteoclastic phenotype induction of the 24 h-FSS CM. The IL- 11 mRNA up-regulation in SaOS-2 cells by the 6 h-FSS was not inhibited by the anti-human transforming growth factor-β1 antibody, but it was significantly inhibited by indomethacin. An enzymeimmunoassay showed prostaglandin E2 to increase 7-fold in the 1 h-FSS CM. These findings thus suggest that FSS induces osteoblasts to produce IL-11 (mediated by prostaglandins) and thus stimulates bone remodeling.

AB - It is unclear how mechanical stress influences bone cells. Mechanical stress causes fluid shear stress (FSS) in the bone. Osteoblast lineage cells are thought to sense FSS and regulate bone remodeling. We therefore investigated the effects of FSS on human osteoblast-like osteosarcoma cells: SaOS-2 cells in vitro. The conditioned medium of the SaOS-2 cells after 24 h of FSS (24 h-FSS CM) showed such osteoclastic phenotype inductions as significantly increasing the number of tartrate-resistant acid phosphatase (TRAP) positive multinuclear cells in rat bone marrow cells and TRAP-positive cells in human preosteoclastic cells: FLG 29.1 cells. An enzyme-linked immunosorbent assay showed interleukin-11 (IL-11) protein to increase 7-fold in the 24 h-FSS CM. A Northern analysis showed that IL-11 mRNA increased 4- fold in the SaOS-2 cells after 6 h-FSS; however, no IL-6 mRNA expression was detected. Furthermore, the anti-human IL-11 antibody significantly neutralized the osteoclastic phenotype induction of the 24 h-FSS CM. The IL- 11 mRNA up-regulation in SaOS-2 cells by the 6 h-FSS was not inhibited by the anti-human transforming growth factor-β1 antibody, but it was significantly inhibited by indomethacin. An enzymeimmunoassay showed prostaglandin E2 to increase 7-fold in the 1 h-FSS CM. These findings thus suggest that FSS induces osteoblasts to produce IL-11 (mediated by prostaglandins) and thus stimulates bone remodeling.

UR - http://www.scopus.com/inward/record.url?scp=0033387265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033387265&partnerID=8YFLogxK

U2 - 10.1359/jbmr.1999.14.12.2089

DO - 10.1359/jbmr.1999.14.12.2089

M3 - Article

VL - 14

SP - 2089

EP - 2098

JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

IS - 12

ER -