Fluidic MEMS based Biosensor for the Detection of Nucleic acids by using Schizophyllan

Takaaki Isoda, Satoshi Hasegawa, Kazuhiro Noguchi, Kana Takamatsu, Fuyumi Itho, Kazuo Sakurai, Taro Kimura, Seiji Shinkai

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3 Citations (Scopus)

Abstract

Recently, a fluid micro electro mechanical system (fluid MEMS), which is composed of a micro pump, mixer, valve, reactor, sensor, an electric circuit, on a chip, is being applied to a biotechnology or a medical analysis. Authors developed a micro sensor mounted on a chip to measure a concentration of one drop of solution 1nL-10 μL) which contained nuclear acids. The form of the micro sensor mounted on the chip was a pair of Cu electrode which was a diameter of 2mm and was a thickness of 10 μm. The sensing function was evaluated by the changes in a concentration of poly(x) (x=adenine, guanine, uracil, and cytosine) solution which was dropped on a micro sensor. To increase a recognization against nucleic acid, various adsorbent was added in a droplet. Especially, high recognition against the nucleic acid was observed with the adsorbent which modified the surface of polystyrene micro beads(l0 μm) using schizophyllan: a kind of polysaccharide. This biosensor recognized a small quantity of total-RNA and m-RNA extracted from the yeast.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalIEEJ Transactions on Sensors and Micromachines
Volume125
Issue number2
DOIs
Publication statusPublished - Jan 1 2005

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All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Isoda, T., Hasegawa, S., Noguchi, K., Takamatsu, K., Itho, F., Sakurai, K., ... Shinkai, S. (2005). Fluidic MEMS based Biosensor for the Detection of Nucleic acids by using Schizophyllan. IEEJ Transactions on Sensors and Micromachines, 125(2), 81-88. https://doi.org/10.1541/ieejsmas.125.81