Fluorescence detection of deoxyadenosine in Cordyceps spp. By indicator displacement assay

Arinta Agnie Dewantari, Nattha Yongwattana, Panwajee Payongsri, Sawinee Seemakhan, Suparerk Borwornpinyo, Akio Ojida, Jirarut Wongkongkatep

Research output: Contribution to journalArticle

Abstract

A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2'- and 3'-deoxyadenosine (2'-dAde and 3'-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated acridine orange (AOH+) and cucurbit[7]uril (CB7) resulted in the hypochromic shift of fluorescent emission from 530 nm to 512 nm. Addition of cordycepin to the highly fluorescent AOH+/CB7 complex resulted in a unique tripartite AOH+/CB7/dAde complex with diminished fluorescence, and such reduction in emission intensity serves as the basis for our novel sensing system. The detection limits were 11 and 82 µM for 2'- and 3'-deoxyadenosine, respectively. The proposed method also demonstrated high selectivity toward 2'- and 3'-deoxyadenosine, owing to the inability of other deoxynucleosides, nucleosides and nucleotides commonly found in Cordyceps spp. to displace the AOH+ from the AOH+/CB7 complex, which was confirmed by isothermal titration calorimetry (ITC), UV-Visible and proton nuclear magnetic resonance (1H-NMR) spectroscopy. Our method was successfully implemented in the analysis of cordycepin in commercially available Ophiocordyceps and Cordyceps supplements, providing a novel and effective tool for quality assessment of these precious fungi with several health benefits.

Original languageEnglish
Article number2045
JournalMolecules
Volume25
Issue number9
DOIs
Publication statusPublished - May 2020

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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  • Cite this

    Dewantari, A. A., Yongwattana, N., Payongsri, P., Seemakhan, S., Borwornpinyo, S., Ojida, A., & Wongkongkatep, J. (2020). Fluorescence detection of deoxyadenosine in Cordyceps spp. By indicator displacement assay. Molecules, 25(9), [2045]. https://doi.org/10.3390/molecules25092045