Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor

Masayasu Taki, Mika Desaki, Akio Ojida, Shohei Iyoshi, Tasuku Hirayama, Itaru Hamachi, Yukio Yamamoto

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.

Original languageEnglish
Pages (from-to)12564-12565
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number38
DOIs
Publication statusPublished - Sep 24 2008
Externally publishedYes

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Optical Imaging
Cadmium
Fluorescence
Imaging techniques
Quantum yield
Metals
Wavelength
Sensors
coumarin

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor. / Taki, Masayasu; Desaki, Mika; Ojida, Akio; Iyoshi, Shohei; Hirayama, Tasuku; Hamachi, Itaru; Yamamoto, Yukio.

In: Journal of the American Chemical Society, Vol. 130, No. 38, 24.09.2008, p. 12564-12565.

Research output: Contribution to journalArticle

Taki, Masayasu ; Desaki, Mika ; Ojida, Akio ; Iyoshi, Shohei ; Hirayama, Tasuku ; Hamachi, Itaru ; Yamamoto, Yukio. / Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor. In: Journal of the American Chemical Society. 2008 ; Vol. 130, No. 38. pp. 12564-12565.
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AU - Yamamoto, Yukio

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