Fluorescence microscopic imaging using a substrate with a sub-wavelength grating

Keiko Tawa, Hironobu Hori, Yoshiro Tatsu, Junji Nishii, Kenji Kintaka, Kazuyuki Kiyosue

Research output: Contribution to journalShort surveypeer-review

Abstract

A study was conducted to demonstrate fluorescent microscopic imaging using a substrate with a sub-wavelength grating. Enhanced fluorescence detection was carried out using grating-coupled (GC)-surface plasmon resonance (SPR) to overcome the challenges associated with conventional prism coupled SPR-design. A thin metal coated grating substrate with a rectangular or trapezoidal surface profile in GC-SPR allowed the resonance angle to be reduced. The periodic structure was fabricated using two-beam interference and dry etching or UV-nanoprint lithography. It was also demonstrated that a periodical structure of 400 nm created on the substrate surface was covered with Ag and SiO 2 layers to be used as a chip.

Original languageEnglish
Pages (from-to)9-10
Number of pages2
JournalAIST Today (International Edition)
Issue number34
Publication statusPublished - 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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