Fluorescent detection of target proteins: Via a molecularly imprinted hydrogel

Takuya Kubo, Naoki Watanabe, Seiji Ikari, Chenchen Liu, Eisuke Kanao, Toyohiro Naito, Tomoharu Sano, Koji Otsuka

Research output: Contribution to journalArticlepeer-review

Abstract

Proteins are typically separated by an immune reaction, such as an enzyme-linked immunosorbent assay, and are detected by selective fluorescent labeling. This has potential for complicated procedures and the denaturation of proteins by labeling, and is cost consuming. In this study, we propose a technique for the selective separation and detection of a target protein using a molecularly imprinted hydrogel (PI gel) with fluorescent monomers. We focused on 8-anilino-1-naphthalenesulfonic acid (ANS), where the fluorescence intensity is easily changed by the interaction with proteins, and successfully synthesized the ANS monomer and a poly(ethylene glycol) (PEG) conjugated ANS monomer. The PI gel with the ANS monomers using bovine serum albumin (BSA) as a template showed the selective adsorption of BSA and the fluorescence intensity increased due to the adsorption of BSA.

Original languageEnglish
Pages (from-to)3086-3091
Number of pages6
JournalAnalytical Methods
Volume13
Issue number27
DOIs
Publication statusPublished - Jul 21 2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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