Fluorescent differential display: Arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer

Takashi Ito, Keiji Kito, Naoki Adati, Yuko Mitsui, Hisashi Hagiwara, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalFEBS Letters
Volume351
Issue number2
DOIs
Publication statusPublished - Sep 5 1994
Externally publishedYes

Fingerprint

Display devices
RNA
Safety
Polymerase Chain Reaction
DNA
Throughput

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Fluorescent differential display : Arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer. / Ito, Takashi; Kito, Keiji; Adati, Naoki; Mitsui, Yuko; Hagiwara, Hisashi; Sakaki, Yoshiyuki.

In: FEBS Letters, Vol. 351, No. 2, 05.09.1994, p. 231-236.

Research output: Contribution to journalArticle

Ito, Takashi ; Kito, Keiji ; Adati, Naoki ; Mitsui, Yuko ; Hagiwara, Hisashi ; Sakaki, Yoshiyuki. / Fluorescent differential display : Arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer. In: FEBS Letters. 1994 ; Vol. 351, No. 2. pp. 231-236.
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