Fluorescent labeling of a DNA sequencing primer

Junichi Mineno, Yoshizumi Ishino, Tadashi Ohminami, Ikunoshin Kato

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were hybridized with less stability than that containing fluorescein at only the 5' terminus, but it formed more efficient primers for dideoxy sequencing with an automated sequencer.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalMitochondrial DNA
Volume4
Issue number3
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

DNA Primers
Fluorescein
DNA Sequence Analysis
Labeling
Oligomers
Oligonucleotides
Labels
Thermodynamic stability
Hot Temperature
Fluorescence
Phosphates
Molecules

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Fluorescent labeling of a DNA sequencing primer. / Mineno, Junichi; Ishino, Yoshizumi; Ohminami, Tadashi; Kato, Ikunoshin.

In: Mitochondrial DNA, Vol. 4, No. 3, 01.01.1993, p. 135-141.

Research output: Contribution to journalArticle

Mineno, Junichi ; Ishino, Yoshizumi ; Ohminami, Tadashi ; Kato, Ikunoshin. / Fluorescent labeling of a DNA sequencing primer. In: Mitochondrial DNA. 1993 ; Vol. 4, No. 3. pp. 135-141.
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