Abstract
Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were hybridized with less stability than that containing fluorescein at only the 5' terminus, but it formed more efficient primers for dideoxy sequencing with an automated sequencer.
| Original language | English |
|---|---|
| Pages (from-to) | 135-141 |
| Number of pages | 7 |
| Journal | Mitochondrial DNA |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Cite this
Fluorescent labeling of a DNA sequencing primer. / Mineno, Junichi; Ishino, Yoshizumi; Ohminami, Tadashi; Kato, Ikunoshin.
In: Mitochondrial DNA, Vol. 4, No. 3, 01.01.1993, p. 135-141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fluorescent labeling of a DNA sequencing primer
AU - Mineno, Junichi
AU - Ishino, Yoshizumi
AU - Ohminami, Tadashi
AU - Kato, Ikunoshin
PY - 1993/1/1
Y1 - 1993/1/1
N2 - Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were hybridized with less stability than that containing fluorescein at only the 5' terminus, but it formed more efficient primers for dideoxy sequencing with an automated sequencer.
AB - Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were hybridized with less stability than that containing fluorescein at only the 5' terminus, but it formed more efficient primers for dideoxy sequencing with an automated sequencer.
UR - http://www.scopus.com/inward/record.url?scp=0027130638&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027130638&partnerID=8YFLogxK
U2 - 10.3109/10425179309015626
DO - 10.3109/10425179309015626
M3 - Article
C2 - 8161816
AN - SCOPUS:0027130638
VL - 4
SP - 135
EP - 141
JO - DNA Sequence - Journal of DNA Sequencing and Mapping
JF - DNA Sequence - Journal of DNA Sequencing and Mapping
SN - 1940-1736
IS - 3
ER -