Abstract
Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were hybridized with less stability than that containing fluorescein at only the 5' terminus, but it formed more efficient primers for dideoxy sequencing with an automated sequencer.
| Original language | English |
|---|---|
| Pages (from-to) | 135-141 |
| Number of pages | 7 |
| Journal | Mitochondrial DNA |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
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Cite this
Mineno, J., Ishino, Y., Ohminami, T., & Kato, I. (1993). Fluorescent labeling of a DNA sequencing primer. Mitochondrial DNA, 4(3), 135-141. https://doi.org/10.3109/10425179309015626